Abstract
Transient forebrain ischemia is associated with selective neuronal vulnerability and persistent memory deficit. This study compares functional outcome and morphological changes in rats subjected to post-ischemic CA1 or hilus/dentate gyrus region hippocampal fetal transplantation. Ischemia was produced by bilateral common carotid artery occlusion with hypotension. Fetal hippocampal neurons were transplanted into both sides of the CA1 or hilus/dentate gyrus region of the dorsal hippocampus, 1 week post-ischemia. Four weeks post transplantation, the rats underwent behavioral testing for 5 consecutive days using the water maze trial. All animals were perfusion fixed for morphological studies. Transplants in the CA1 region of the dorsal hippocampus were associated with memory and morphological recovery, while grafts placed into the hilus/dentate gyrus region of the dorsal hippocampus were not. Similarly, neurons transplanted in the CA1 region of the dorsal hippocampus were morphologically similar to CA1 pyramidal cell neurons and stained positive with calbindin D(28k). In contrast the grafts transplanted into the hilus/dentate gyrus region of the dorsal hippocampus were morphologically heterogeneous and staining with calbindin D(28k) was not as robust. Post-ischemic transplantation in the CA1 region of the dorsal hippocampus is effective in improving memory and morphological function.
| Original language | English |
|---|---|
| Pages (from-to) | 249-255 |
| Number of pages | 7 |
| Journal | Neuroscience Research |
| Volume | 27 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 1997 |
| Externally published | Yes |
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Keywords
- Fetal neurons
- Hippocampus
- Ischemia
- Spatial memory
- Transplantation
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Embryonic transplantation and ischemic memory deficit. / Masada, Tetsuya; Itano, Toshifumi; Fujisawa, Mutsuo; Miyamoto, Osamu; Tokuda, Masaaki; Matsui, Hideki; Nagao, Seigo; Hatase, Osamu.
In: Neuroscience Research, Vol. 27, No. 3, 03.1997, p. 249-255.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Embryonic transplantation and ischemic memory deficit
AU - Masada, Tetsuya
AU - Itano, Toshifumi
AU - Fujisawa, Mutsuo
AU - Miyamoto, Osamu
AU - Tokuda, Masaaki
AU - Matsui, Hideki
AU - Nagao, Seigo
AU - Hatase, Osamu
PY - 1997/3
Y1 - 1997/3
N2 - Transient forebrain ischemia is associated with selective neuronal vulnerability and persistent memory deficit. This study compares functional outcome and morphological changes in rats subjected to post-ischemic CA1 or hilus/dentate gyrus region hippocampal fetal transplantation. Ischemia was produced by bilateral common carotid artery occlusion with hypotension. Fetal hippocampal neurons were transplanted into both sides of the CA1 or hilus/dentate gyrus region of the dorsal hippocampus, 1 week post-ischemia. Four weeks post transplantation, the rats underwent behavioral testing for 5 consecutive days using the water maze trial. All animals were perfusion fixed for morphological studies. Transplants in the CA1 region of the dorsal hippocampus were associated with memory and morphological recovery, while grafts placed into the hilus/dentate gyrus region of the dorsal hippocampus were not. Similarly, neurons transplanted in the CA1 region of the dorsal hippocampus were morphologically similar to CA1 pyramidal cell neurons and stained positive with calbindin D(28k). In contrast the grafts transplanted into the hilus/dentate gyrus region of the dorsal hippocampus were morphologically heterogeneous and staining with calbindin D(28k) was not as robust. Post-ischemic transplantation in the CA1 region of the dorsal hippocampus is effective in improving memory and morphological function.
AB - Transient forebrain ischemia is associated with selective neuronal vulnerability and persistent memory deficit. This study compares functional outcome and morphological changes in rats subjected to post-ischemic CA1 or hilus/dentate gyrus region hippocampal fetal transplantation. Ischemia was produced by bilateral common carotid artery occlusion with hypotension. Fetal hippocampal neurons were transplanted into both sides of the CA1 or hilus/dentate gyrus region of the dorsal hippocampus, 1 week post-ischemia. Four weeks post transplantation, the rats underwent behavioral testing for 5 consecutive days using the water maze trial. All animals were perfusion fixed for morphological studies. Transplants in the CA1 region of the dorsal hippocampus were associated with memory and morphological recovery, while grafts placed into the hilus/dentate gyrus region of the dorsal hippocampus were not. Similarly, neurons transplanted in the CA1 region of the dorsal hippocampus were morphologically similar to CA1 pyramidal cell neurons and stained positive with calbindin D(28k). In contrast the grafts transplanted into the hilus/dentate gyrus region of the dorsal hippocampus were morphologically heterogeneous and staining with calbindin D(28k) was not as robust. Post-ischemic transplantation in the CA1 region of the dorsal hippocampus is effective in improving memory and morphological function.
KW - Fetal neurons
KW - Hippocampus
KW - Ischemia
KW - Spatial memory
KW - Transplantation
UR - http://www.scopus.com/inward/record.url?scp=0030975304&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030975304&partnerID=8YFLogxK
U2 - 10.1016/S0168-0102(97)01158-9
DO - 10.1016/S0168-0102(97)01158-9
M3 - Article
VL - 27
SP - 249
EP - 255
JO - Neuroscience Research
JF - Neuroscience Research
SN - 0168-0102
IS - 3
ER -