Embryonic expression of pericentrin suggests universal roles in ciliogenesis

Ko Miyoshi, Kazunari Onishi, Masato Asanuma, Ikuko Miyazaki, Francisco J. Diaz-Corrales, Norio Ogawa

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Pericentrin (Pcnt) is a giant coiled-coil protein known to mediate microtubule organization. It has been recently reported that mitosis-specific centrosomal anchoring of γ tubulin complexes by Pcnt acts to control mitotic spindle organization, though little is known about the in vivo expression of Pcnt. In this study, we investigated Pcnt expression in mouse embryos. In situ hybridization analysis revealed preferential expression of Pcnt in quiescent G0 phase cells throughout the embryo with an unexpectedly low expression level in proliferating cells, suggesting that Pcnt might not play an important role in mitotic proliferation. Immunofluorescence analysis confirmed preferential expression of the Pcnt protein in G0 phase cells. Moreover, Pcnt was shown to be localized to the base of primary cilia in multiple embryonic tissues, in agreement with a recent study demonstrating the involvement of Pcnt in primary cilia formation using cultured mammalian cells.

Original languageEnglish
Pages (from-to)537-542
Number of pages6
JournalDevelopment Genes and Evolution
Volume216
Issue number9
DOIs
Publication statusPublished - Sep 2006

Fingerprint

embryo
protein
cilia
interphase
cells
mitotic spindle apparatus
Cell Cycle Resting Phase
Cilia
tubulin
mitosis
in situ hybridization
microtubules
fluorescent antibody technique
protein synthesis
Embryonic Structures
analysis
pericentrin
mice
Spindle Apparatus
Tubulin

Keywords

  • Basal body
  • Cilium
  • Differentiation
  • Kendrin
  • Pericentrin

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Genetics
  • Ecology, Evolution, Behavior and Systematics

Cite this

Embryonic expression of pericentrin suggests universal roles in ciliogenesis. / Miyoshi, Ko; Onishi, Kazunari; Asanuma, Masato; Miyazaki, Ikuko; Diaz-Corrales, Francisco J.; Ogawa, Norio.

In: Development Genes and Evolution, Vol. 216, No. 9, 09.2006, p. 537-542.

Research output: Contribution to journalArticle

Miyoshi, Ko ; Onishi, Kazunari ; Asanuma, Masato ; Miyazaki, Ikuko ; Diaz-Corrales, Francisco J. ; Ogawa, Norio. / Embryonic expression of pericentrin suggests universal roles in ciliogenesis. In: Development Genes and Evolution. 2006 ; Vol. 216, No. 9. pp. 537-542.
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