Abstract
Enzyme-linked immunosorbent assay (ELISA) is the most popular methodology for absolute quantification of particular proteins in liquid samples. Especially for CCN family members that are associated with human diseases, utility of ELISA for those proteins in clinics as well as research laboratories is emphasized. However, in order to obtain accurate and stable results in ELISA, particular care should be taken in controlling the quality and quantity of standard CCN family proteins, which bind to various materials and can be unstable in a purified form. Recently, diagnostic value of the CCN family protein fragments in body fluids has been indicated in several diseases. Therefore, module-specific detection system for the CCN family members is desired as a promising tool in clinics. It should be also noted that modular fragments of CCN family members can be more stable than the full-length in purified forms, whose quality may be easier to control than that of the full-length ones.
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 127-138 |
| Number of pages | 12 |
| Volume | 1489 |
| DOIs | |
| Publication status | Published - 2017 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1489 |
| ISSN (Print) | 10643745 |
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Keywords
- CCN family
- ELISA
- Fibrosis
- Platelet
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Elisa of CCN family proteins in body fluids including serum and plasma. / Kubota, Satoshi; Kawaki, Harumi; Takigawa, Masaharu.
Methods in Molecular Biology. Vol. 1489 Humana Press Inc., 2017. p. 127-138 (Methods in Molecular Biology; Vol. 1489).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Elisa of CCN family proteins in body fluids including serum and plasma
AU - Kubota, Satoshi
AU - Kawaki, Harumi
AU - Takigawa, Masaharu
PY - 2017
Y1 - 2017
N2 - Enzyme-linked immunosorbent assay (ELISA) is the most popular methodology for absolute quantification of particular proteins in liquid samples. Especially for CCN family members that are associated with human diseases, utility of ELISA for those proteins in clinics as well as research laboratories is emphasized. However, in order to obtain accurate and stable results in ELISA, particular care should be taken in controlling the quality and quantity of standard CCN family proteins, which bind to various materials and can be unstable in a purified form. Recently, diagnostic value of the CCN family protein fragments in body fluids has been indicated in several diseases. Therefore, module-specific detection system for the CCN family members is desired as a promising tool in clinics. It should be also noted that modular fragments of CCN family members can be more stable than the full-length in purified forms, whose quality may be easier to control than that of the full-length ones.
AB - Enzyme-linked immunosorbent assay (ELISA) is the most popular methodology for absolute quantification of particular proteins in liquid samples. Especially for CCN family members that are associated with human diseases, utility of ELISA for those proteins in clinics as well as research laboratories is emphasized. However, in order to obtain accurate and stable results in ELISA, particular care should be taken in controlling the quality and quantity of standard CCN family proteins, which bind to various materials and can be unstable in a purified form. Recently, diagnostic value of the CCN family protein fragments in body fluids has been indicated in several diseases. Therefore, module-specific detection system for the CCN family members is desired as a promising tool in clinics. It should be also noted that modular fragments of CCN family members can be more stable than the full-length in purified forms, whose quality may be easier to control than that of the full-length ones.
KW - CCN family
KW - ELISA
KW - Fibrosis
KW - Platelet
UR - http://www.scopus.com/inward/record.url?scp=84991737451&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84991737451&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-6430-7_13
DO - 10.1007/978-1-4939-6430-7_13
M3 - Chapter
VL - 1489
T3 - Methods in Molecular Biology
SP - 127
EP - 138
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -