Elisa of CCN family proteins in body fluids including serum and plasma

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Enzyme-linked immunosorbent assay (ELISA) is the most popular methodology for absolute quantification of particular proteins in liquid samples. Especially for CCN family members that are associated with human diseases, utility of ELISA for those proteins in clinics as well as research laboratories is emphasized. However, in order to obtain accurate and stable results in ELISA, particular care should be taken in controlling the quality and quantity of standard CCN family proteins, which bind to various materials and can be unstable in a purified form. Recently, diagnostic value of the CCN family protein fragments in body fluids has been indicated in several diseases. Therefore, module-specific detection system for the CCN family members is desired as a promising tool in clinics. It should be also noted that modular fragments of CCN family members can be more stable than the full-length in purified forms, whose quality may be easier to control than that of the full-length ones.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages127-138
Number of pages12
Volume1489
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1489
ISSN (Print)10643745

Fingerprint

CCN Intercellular Signaling Proteins
Body Fluids
Enzyme-Linked Immunosorbent Assay
Serum
Proteins
Research

Keywords

  • CCN family
  • ELISA
  • Fibrosis
  • Platelet

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Kubota, S., Kawaki, H., & Takigawa, M. (2017). Elisa of CCN family proteins in body fluids including serum and plasma. In Methods in Molecular Biology (Vol. 1489, pp. 127-138). (Methods in Molecular Biology; Vol. 1489). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6430-7_13

Elisa of CCN family proteins in body fluids including serum and plasma. / Kubota, Satoshi; Kawaki, Harumi; Takigawa, Masaharu.

Methods in Molecular Biology. Vol. 1489 Humana Press Inc., 2017. p. 127-138 (Methods in Molecular Biology; Vol. 1489).

Research output: Chapter in Book/Report/Conference proceedingChapter

Kubota, S, Kawaki, H & Takigawa, M 2017, Elisa of CCN family proteins in body fluids including serum and plasma. in Methods in Molecular Biology. vol. 1489, Methods in Molecular Biology, vol. 1489, Humana Press Inc., pp. 127-138. https://doi.org/10.1007/978-1-4939-6430-7_13
Kubota S, Kawaki H, Takigawa M. Elisa of CCN family proteins in body fluids including serum and plasma. In Methods in Molecular Biology. Vol. 1489. Humana Press Inc. 2017. p. 127-138. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6430-7_13
Kubota, Satoshi ; Kawaki, Harumi ; Takigawa, Masaharu. / Elisa of CCN family proteins in body fluids including serum and plasma. Methods in Molecular Biology. Vol. 1489 Humana Press Inc., 2017. pp. 127-138 (Methods in Molecular Biology).
@inbook{051dac53f3bb4c438a1c9105e27a5c95,
title = "Elisa of CCN family proteins in body fluids including serum and plasma",
abstract = "Enzyme-linked immunosorbent assay (ELISA) is the most popular methodology for absolute quantification of particular proteins in liquid samples. Especially for CCN family members that are associated with human diseases, utility of ELISA for those proteins in clinics as well as research laboratories is emphasized. However, in order to obtain accurate and stable results in ELISA, particular care should be taken in controlling the quality and quantity of standard CCN family proteins, which bind to various materials and can be unstable in a purified form. Recently, diagnostic value of the CCN family protein fragments in body fluids has been indicated in several diseases. Therefore, module-specific detection system for the CCN family members is desired as a promising tool in clinics. It should be also noted that modular fragments of CCN family members can be more stable than the full-length in purified forms, whose quality may be easier to control than that of the full-length ones.",
keywords = "CCN family, ELISA, Fibrosis, Platelet",
author = "Satoshi Kubota and Harumi Kawaki and Masaharu Takigawa",
year = "2017",
doi = "10.1007/978-1-4939-6430-7_13",
language = "English",
volume = "1489",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "127--138",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Elisa of CCN family proteins in body fluids including serum and plasma

AU - Kubota, Satoshi

AU - Kawaki, Harumi

AU - Takigawa, Masaharu

PY - 2017

Y1 - 2017

N2 - Enzyme-linked immunosorbent assay (ELISA) is the most popular methodology for absolute quantification of particular proteins in liquid samples. Especially for CCN family members that are associated with human diseases, utility of ELISA for those proteins in clinics as well as research laboratories is emphasized. However, in order to obtain accurate and stable results in ELISA, particular care should be taken in controlling the quality and quantity of standard CCN family proteins, which bind to various materials and can be unstable in a purified form. Recently, diagnostic value of the CCN family protein fragments in body fluids has been indicated in several diseases. Therefore, module-specific detection system for the CCN family members is desired as a promising tool in clinics. It should be also noted that modular fragments of CCN family members can be more stable than the full-length in purified forms, whose quality may be easier to control than that of the full-length ones.

AB - Enzyme-linked immunosorbent assay (ELISA) is the most popular methodology for absolute quantification of particular proteins in liquid samples. Especially for CCN family members that are associated with human diseases, utility of ELISA for those proteins in clinics as well as research laboratories is emphasized. However, in order to obtain accurate and stable results in ELISA, particular care should be taken in controlling the quality and quantity of standard CCN family proteins, which bind to various materials and can be unstable in a purified form. Recently, diagnostic value of the CCN family protein fragments in body fluids has been indicated in several diseases. Therefore, module-specific detection system for the CCN family members is desired as a promising tool in clinics. It should be also noted that modular fragments of CCN family members can be more stable than the full-length in purified forms, whose quality may be easier to control than that of the full-length ones.

KW - CCN family

KW - ELISA

KW - Fibrosis

KW - Platelet

UR - http://www.scopus.com/inward/record.url?scp=84991737451&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84991737451&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-6430-7_13

DO - 10.1007/978-1-4939-6430-7_13

M3 - Chapter

VL - 1489

T3 - Methods in Molecular Biology

SP - 127

EP - 138

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -