Elicitin-responsive lectin-like receptor kinase genes in BY-2 cells

Michiko Sasabe, Kana Naito, Hiroko Suenaga, Takako Ikeda, Kazuhiro Toyoda, Yoshishige Inagaki, Tomonori Shiraishi, Yuki Ichinose

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The inhibition of elicitor-induced plant defense responses by the protein kinase inhibitors K252a and staurosporine indicates that defense responses require protein phosphorylation. We isolated a cDNA clone encoding Nicotiana tabacum lectin-like receptor protein kinase 1 (NtlecRK1), an elicitor-responsive gene; in tobacco bright yellow (BY-2) cells by a differential display method. NtlecRK forms a gene family with at least three members in tobacco. All three NtlecRK genes potentially encode the N-terminal legume lectin domain, transmembrane domain and C-terminal Ser/Thr-type protein kinase domain. Green fluorescent protein (GFP) fusion showed that the NtlecRK1 protein was located on the plasma membrane. In addition, NtlecRK1 and 3 were responsive to INF1 elicitin and the bacterial elicitor harpin. These results indicate that NtlecRKs are membrane-located protein kinases that are induced during defense responses in BY-2 cells.

Original languageEnglish
Pages (from-to)152-159
Number of pages8
JournalDNA Sequence - Journal of DNA Sequencing and Mapping
Volume18
Issue number2
DOIs
Publication statusPublished - 2007

Fingerprint

Mitogen Receptors
Lectins
Protein Kinases
Tobacco
Phosphotransferases
Genes
Phosphorylation
Staurosporine
Cell membranes
Protein Kinase Inhibitors
Green Fluorescent Proteins
Fabaceae
Membrane Proteins
Proteins
Fusion reactions
Complementary DNA
Clone Cells
Display devices
Cell Membrane
Membranes

Keywords

  • Defense response
  • Elicitin
  • Lectin
  • Receptor-like kinase
  • Tobacco BY-2

ASJC Scopus subject areas

  • Genetics
  • Biochemistry
  • Endocrinology
  • Molecular Biology

Cite this

Elicitin-responsive lectin-like receptor kinase genes in BY-2 cells. / Sasabe, Michiko; Naito, Kana; Suenaga, Hiroko; Ikeda, Takako; Toyoda, Kazuhiro; Inagaki, Yoshishige; Shiraishi, Tomonori; Ichinose, Yuki.

In: DNA Sequence - Journal of DNA Sequencing and Mapping, Vol. 18, No. 2, 2007, p. 152-159.

Research output: Contribution to journalArticle

Sasabe, Michiko ; Naito, Kana ; Suenaga, Hiroko ; Ikeda, Takako ; Toyoda, Kazuhiro ; Inagaki, Yoshishige ; Shiraishi, Tomonori ; Ichinose, Yuki. / Elicitin-responsive lectin-like receptor kinase genes in BY-2 cells. In: DNA Sequence - Journal of DNA Sequencing and Mapping. 2007 ; Vol. 18, No. 2. pp. 152-159.
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