Elevation of mouse liver glutathione level by low-dose γ-ray irradiation and its effect on CCl4-induced liver damage

Shuji Kojima, Osamu Matsuki, Takaharu Nomura, Akiko Kubodera, Kiyonori Yamaoka

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We examined the elevation of glutathione (GSH) levels in the liver of C57BL/6 female mice after low-dose γ-ray irradiation and its inhibitory effect on CCl4-induced liver damage. The liver GSH level increased soon after irradiation with 50 cGy of γ-rays, reached a maximum at around 12 post-treatment, and returned almost to the control level by 24 h. The activities of glutathione reductase, and glutathione peroxidase also showed the same pattern of change, while the activity of γ-glutamylcysteine synthetase showed a gradual increase up to 24 h. The effect of pre-irradiation on CCl4-induced liver damage was also investigated. The activities of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase in serum were markedly increased 12 h post-treatment with CCl4. Both increases were significantly suppressed by a single low-dose pre-irradiation. Malondialdehyde, a marker of lipidperoxidation, was also greatly elevated after CCl4 treatment, and its increase was suppressed by irradiation. These results suggest low-dose γ-ray irradiation might be effective for the prevention of and/or therapy of various reactive oxygen species-related diseases including cancer.

Original languageEnglish
Pages (from-to)2471-2476
Number of pages6
JournalAnticancer research
Volume18
Issue number4 A
Publication statusPublished - Jul 1 1998
Externally publishedYes

Keywords

  • Glutathione
  • Liver damage
  • Low-dose γ-ray

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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