Elevation of interleukin-6 in inflammatory bowel disease is macrophage- and epithelial cell-dependent

Kazuo Kusugami, Atsushi Fukatsu, Mitsune Tanimoto, Masataka Shinoda, Jun Ichi Haruta, Atsuo Kuroiwa, Kenji Ina, Kazuhiro Kanayama, Takafumi Ando, Toshihiro Matsuura, Takeo Yamaguchi, Kimitomo Morise, Mayumi Ieda, Hitoshi Iokawa, Akira Ishihara, Susumu Sarai

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

Local interleukin-6 (IL-6) activity was studied using colonic mucosal tissues in inflammatory bowel disease (IBD) and inflammatory control patients. Active IBD specimens exhibited significantly higher IL-6 activity than control specimens in both cultures of isolated lamina propria mononuclear cells (LPMC) and mucosal tissues with an increased number of IL-6-producing cells. However, the activity in inactive IBD or inflammatory controls did not differ from controls. Northern blot analysis demonstrated IL-6 messenger RNA in LPMC and colonic epithelial cells isolated from active IBD specimens but not in control cells. Furthermore, immunofluorescent microscopic study of active IBD specimens showed more conspicuous staining of IL-6 in infiltrating LPMC (mostly CD68+ cells) and colonic epithelial cells. These results suggest that elevation of local IL-6 activity may be a characteristic feature of active IBD and both macrophages and colonic epithelial cells are the major cell types responsible for this phenomenon.

Original languageEnglish
Pages (from-to)949-959
Number of pages11
JournalDigestive Diseases and Sciences
Volume40
Issue number5
DOIs
Publication statusPublished - May 1995
Externally publishedYes

Fingerprint

Inflammatory Bowel Diseases
Interleukin-6
Epithelial Cells
Macrophages
Mucous Membrane
Northern Blotting
Staining and Labeling
Messenger RNA

Keywords

  • colonic epithelial cells
  • inflammatory bowel disease
  • interleukin-6
  • macrophages

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Kusugami, K., Fukatsu, A., Tanimoto, M., Shinoda, M., Haruta, J. I., Kuroiwa, A., ... Sarai, S. (1995). Elevation of interleukin-6 in inflammatory bowel disease is macrophage- and epithelial cell-dependent. Digestive Diseases and Sciences, 40(5), 949-959. https://doi.org/10.1007/BF02064182

Elevation of interleukin-6 in inflammatory bowel disease is macrophage- and epithelial cell-dependent. / Kusugami, Kazuo; Fukatsu, Atsushi; Tanimoto, Mitsune; Shinoda, Masataka; Haruta, Jun Ichi; Kuroiwa, Atsuo; Ina, Kenji; Kanayama, Kazuhiro; Ando, Takafumi; Matsuura, Toshihiro; Yamaguchi, Takeo; Morise, Kimitomo; Ieda, Mayumi; Iokawa, Hitoshi; Ishihara, Akira; Sarai, Susumu.

In: Digestive Diseases and Sciences, Vol. 40, No. 5, 05.1995, p. 949-959.

Research output: Contribution to journalArticle

Kusugami, K, Fukatsu, A, Tanimoto, M, Shinoda, M, Haruta, JI, Kuroiwa, A, Ina, K, Kanayama, K, Ando, T, Matsuura, T, Yamaguchi, T, Morise, K, Ieda, M, Iokawa, H, Ishihara, A & Sarai, S 1995, 'Elevation of interleukin-6 in inflammatory bowel disease is macrophage- and epithelial cell-dependent', Digestive Diseases and Sciences, vol. 40, no. 5, pp. 949-959. https://doi.org/10.1007/BF02064182
Kusugami, Kazuo ; Fukatsu, Atsushi ; Tanimoto, Mitsune ; Shinoda, Masataka ; Haruta, Jun Ichi ; Kuroiwa, Atsuo ; Ina, Kenji ; Kanayama, Kazuhiro ; Ando, Takafumi ; Matsuura, Toshihiro ; Yamaguchi, Takeo ; Morise, Kimitomo ; Ieda, Mayumi ; Iokawa, Hitoshi ; Ishihara, Akira ; Sarai, Susumu. / Elevation of interleukin-6 in inflammatory bowel disease is macrophage- and epithelial cell-dependent. In: Digestive Diseases and Sciences. 1995 ; Vol. 40, No. 5. pp. 949-959.
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