We examined the relationship between the induction of an increase in the level of glutathione and the elevation of natural killer (NK) activity in mouse splenocytes by a low dose of γ rays. The glutathione levels in mouse splenocytes increased significantly between 2 h and 6 h after whole-body γ irradiation at 0.5 Gy, peaked at 4 h, and then decreased almost to the level before irradiation by 12 h postirradiation. A significant enhancement of NK activity was found in the splenocytes obtained from whole-body-irradiated mice between 4 and 6 h postirradiation. Reduced glutathione (GSH) added exogenously to splenocytes obtained from normal mice enhanced both the total cellular glutathione content and the NK activity in a dosedependent manner. Other precursors of de nava GSH synthesis, such as cysteine, N-acetylcysteine and oxidized glutathione, also increased the activity. These enhancements were completely blocked by buthionine sulfoximine, an inhibitor of de nava GSH synthesis. We conclude that the induction of endogenous glutathione in living cells immediately after low-dose γirradiation is at least partially responsible for the appearance of enhanced NK activity.
|Number of pages||6|
|Publication status||Published - Jan 1 2002|
ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging