Elevation of antioxidant potency in the brain of mice by low-dose γ- ray irradiation and its effect on 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP)-induced brain damage

Shuji Kojima, Osamu Matsuki, Takaharu Nomura, Kiyonori Yamaoka, Marekiyo Takahashi, Etsuo Niki

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

The elevation of endogenous thiol-related antioxidants and free radical scavenging enzymes in the brain of C57BL/6 female mice after low-dose γ-ray irradiation and its inhibitory effect on 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP)-induced brain damage were investigated. The brain level of the reduced form of glutathione (GSH) increased soon after irradiation with 50 cGy of γ-rays, reached a maximum at 3 h post-treatment, and remained elevated until 12 h. Thioredoxin (TRX) was also transiently increased after irradiation. The activities of free radical scavenging enzymes, including Cu/Zn-superoxide dismutase, catalase and glutathione peroxidase, were significantly induced after irradiation as well. Cerebral malondialdehyde was remarkably elevated by MPTP treatment, and this elevation was suppressed by pre- irradiation (50 cGy). The contents of GSH and TRX were significantly decreased by MPTP treatment in comparison with those of the control group. These reductions both seemed to be attenuated by pre- irradiation with γ-rays. These results suggest that low-dose γ-ray irradiation induces endogenous antioxidative potency in the brain of mice and might be effective for the prevention and/or therapy of various reactive oxygen species-related neurodegenerative disorders, such as Parkinson's disease and Alzheimer's disease.

Original languageEnglish
Pages (from-to)388-395
Number of pages8
JournalFree Radical Biology and Medicine
Volume26
Issue number3-4
DOIs
Publication statusPublished - Feb 1999
Externally publishedYes

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1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
Dosimetry
Brain
Antioxidants
Irradiation
Thioredoxins
Free Radicals
Scavenging
Enzymes
Glutathione Peroxidase
Malondialdehyde
Sulfhydryl Compounds
Neurodegenerative Diseases
Catalase
Glutathione
Parkinson Disease
Reactive Oxygen Species
Alzheimer Disease
Control Groups
Therapeutics

Keywords

  • γ-Ray
  • Free radical
  • Glutathione
  • Mouse brain
  • MPTP
  • Neurodegenerative disorders
  • Thioredoxin

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

Elevation of antioxidant potency in the brain of mice by low-dose γ- ray irradiation and its effect on 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP)-induced brain damage. / Kojima, Shuji; Matsuki, Osamu; Nomura, Takaharu; Yamaoka, Kiyonori; Takahashi, Marekiyo; Niki, Etsuo.

In: Free Radical Biology and Medicine, Vol. 26, No. 3-4, 02.1999, p. 388-395.

Research output: Contribution to journalArticle

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