Elevated levels of monocyte chemoattractant protein-1 in the aqueous humor after phacoemulsification

Motofumi Kawai, Toshihiro Inoue, Masaru Inatani, Naoko Tsuboi, Kohei Shobayashi, Akihiro Matsukawa, Akitoshi Yoshida, Hidenobu Tanihara

Research output: Contribution to journalArticle

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Abstract

Purpose. To elucidate the impact of phacoemulsification on aqueous monocyte chemoattractant protein-1 (MCP-1) levels, and identify its cell origin. Methods. For clinical study, aqueous humor samples were collected before and after surgery (17.0 6 4.0 months postoperatively) from 21 cataract cases that underwent phacoemulsification and intraocular lens (IOL) implantation. Aqueous MCP-1 levels were determined using a multiplex immunoassay. For animal experiments, rabbits underwent phacoemulsification (6IOL). Aqueous humor samples were collected from nonoperated eyes and operated eyes, and immunoassays were performed. Eyes were analyzed by reverse transcription-polymerase chain reaction and immunohisto-chemical studies. Results. In the clinical study, mean (6SD) aqueous MCP-1 levels were higher postoperatively (1773.5 6 321.2 pg/mL) than preoperatively (796.9 6 211.3 pg/mL; P <0.0001). In animal experiments, mean aqueous MCP-1 levels (pg/mL) were higher in postoperative eyes on day 30 (207.1 6 62.9) than in nonoperated eyes (31.2 6 12.5; P 1/4 0.018). IOL implantation did not affect the changes in MCP-1 levels. After phacoemul-sification, MCP-1 mRNA expression was increased in the cornea, iris, ciliary body, and capsular bag. Expression of MCP-1 mRNA in the capsular bag, but not the other tissues, increased from day 30 to 90. Immunohistochemical studies showed positive immunoreactivity for MCP-1 in cells of the posterior capsule after phacoemulsification. Conclusions. Aqueous MCP-1 levels were elevated in both human and animal eyes after phacoemulsification. Proliferated lens epithelial cells on the capsule might be the major cell origin for prolonged MCP-1 production after phacoemulsifica-tion.

Original languageEnglish
Pages (from-to)7951-7960
Number of pages10
JournalInvestigative Ophthalmology and Visual Science
Volume53
Issue number13
DOIs
Publication statusPublished - Dec 2012

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Phacoemulsification
Aqueous Humor
Chemokine CCL2
Intraocular Lens Implantation
Immunoassay
Capsules
Messenger RNA
Ciliary Body
Iris
Cornea
Cataract
Lenses
Reverse Transcription
Epithelial Cells
Rabbits
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Elevated levels of monocyte chemoattractant protein-1 in the aqueous humor after phacoemulsification. / Kawai, Motofumi; Inoue, Toshihiro; Inatani, Masaru; Tsuboi, Naoko; Shobayashi, Kohei; Matsukawa, Akihiro; Yoshida, Akitoshi; Tanihara, Hidenobu.

In: Investigative Ophthalmology and Visual Science, Vol. 53, No. 13, 12.2012, p. 7951-7960.

Research output: Contribution to journalArticle

Kawai, Motofumi ; Inoue, Toshihiro ; Inatani, Masaru ; Tsuboi, Naoko ; Shobayashi, Kohei ; Matsukawa, Akihiro ; Yoshida, Akitoshi ; Tanihara, Hidenobu. / Elevated levels of monocyte chemoattractant protein-1 in the aqueous humor after phacoemulsification. In: Investigative Ophthalmology and Visual Science. 2012 ; Vol. 53, No. 13. pp. 7951-7960.
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abstract = "Purpose. To elucidate the impact of phacoemulsification on aqueous monocyte chemoattractant protein-1 (MCP-1) levels, and identify its cell origin. Methods. For clinical study, aqueous humor samples were collected before and after surgery (17.0 6 4.0 months postoperatively) from 21 cataract cases that underwent phacoemulsification and intraocular lens (IOL) implantation. Aqueous MCP-1 levels were determined using a multiplex immunoassay. For animal experiments, rabbits underwent phacoemulsification (6IOL). Aqueous humor samples were collected from nonoperated eyes and operated eyes, and immunoassays were performed. Eyes were analyzed by reverse transcription-polymerase chain reaction and immunohisto-chemical studies. Results. In the clinical study, mean (6SD) aqueous MCP-1 levels were higher postoperatively (1773.5 6 321.2 pg/mL) than preoperatively (796.9 6 211.3 pg/mL; P <0.0001). In animal experiments, mean aqueous MCP-1 levels (pg/mL) were higher in postoperative eyes on day 30 (207.1 6 62.9) than in nonoperated eyes (31.2 6 12.5; P 1/4 0.018). IOL implantation did not affect the changes in MCP-1 levels. After phacoemul-sification, MCP-1 mRNA expression was increased in the cornea, iris, ciliary body, and capsular bag. Expression of MCP-1 mRNA in the capsular bag, but not the other tissues, increased from day 30 to 90. Immunohistochemical studies showed positive immunoreactivity for MCP-1 in cells of the posterior capsule after phacoemulsification. Conclusions. Aqueous MCP-1 levels were elevated in both human and animal eyes after phacoemulsification. Proliferated lens epithelial cells on the capsule might be the major cell origin for prolonged MCP-1 production after phacoemulsifica-tion.",
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T1 - Elevated levels of monocyte chemoattractant protein-1 in the aqueous humor after phacoemulsification

AU - Kawai, Motofumi

AU - Inoue, Toshihiro

AU - Inatani, Masaru

AU - Tsuboi, Naoko

AU - Shobayashi, Kohei

AU - Matsukawa, Akihiro

AU - Yoshida, Akitoshi

AU - Tanihara, Hidenobu

PY - 2012/12

Y1 - 2012/12

N2 - Purpose. To elucidate the impact of phacoemulsification on aqueous monocyte chemoattractant protein-1 (MCP-1) levels, and identify its cell origin. Methods. For clinical study, aqueous humor samples were collected before and after surgery (17.0 6 4.0 months postoperatively) from 21 cataract cases that underwent phacoemulsification and intraocular lens (IOL) implantation. Aqueous MCP-1 levels were determined using a multiplex immunoassay. For animal experiments, rabbits underwent phacoemulsification (6IOL). Aqueous humor samples were collected from nonoperated eyes and operated eyes, and immunoassays were performed. Eyes were analyzed by reverse transcription-polymerase chain reaction and immunohisto-chemical studies. Results. In the clinical study, mean (6SD) aqueous MCP-1 levels were higher postoperatively (1773.5 6 321.2 pg/mL) than preoperatively (796.9 6 211.3 pg/mL; P <0.0001). In animal experiments, mean aqueous MCP-1 levels (pg/mL) were higher in postoperative eyes on day 30 (207.1 6 62.9) than in nonoperated eyes (31.2 6 12.5; P 1/4 0.018). IOL implantation did not affect the changes in MCP-1 levels. After phacoemul-sification, MCP-1 mRNA expression was increased in the cornea, iris, ciliary body, and capsular bag. Expression of MCP-1 mRNA in the capsular bag, but not the other tissues, increased from day 30 to 90. Immunohistochemical studies showed positive immunoreactivity for MCP-1 in cells of the posterior capsule after phacoemulsification. Conclusions. Aqueous MCP-1 levels were elevated in both human and animal eyes after phacoemulsification. Proliferated lens epithelial cells on the capsule might be the major cell origin for prolonged MCP-1 production after phacoemulsifica-tion.

AB - Purpose. To elucidate the impact of phacoemulsification on aqueous monocyte chemoattractant protein-1 (MCP-1) levels, and identify its cell origin. Methods. For clinical study, aqueous humor samples were collected before and after surgery (17.0 6 4.0 months postoperatively) from 21 cataract cases that underwent phacoemulsification and intraocular lens (IOL) implantation. Aqueous MCP-1 levels were determined using a multiplex immunoassay. For animal experiments, rabbits underwent phacoemulsification (6IOL). Aqueous humor samples were collected from nonoperated eyes and operated eyes, and immunoassays were performed. Eyes were analyzed by reverse transcription-polymerase chain reaction and immunohisto-chemical studies. Results. In the clinical study, mean (6SD) aqueous MCP-1 levels were higher postoperatively (1773.5 6 321.2 pg/mL) than preoperatively (796.9 6 211.3 pg/mL; P <0.0001). In animal experiments, mean aqueous MCP-1 levels (pg/mL) were higher in postoperative eyes on day 30 (207.1 6 62.9) than in nonoperated eyes (31.2 6 12.5; P 1/4 0.018). IOL implantation did not affect the changes in MCP-1 levels. After phacoemul-sification, MCP-1 mRNA expression was increased in the cornea, iris, ciliary body, and capsular bag. Expression of MCP-1 mRNA in the capsular bag, but not the other tissues, increased from day 30 to 90. Immunohistochemical studies showed positive immunoreactivity for MCP-1 in cells of the posterior capsule after phacoemulsification. Conclusions. Aqueous MCP-1 levels were elevated in both human and animal eyes after phacoemulsification. Proliferated lens epithelial cells on the capsule might be the major cell origin for prolonged MCP-1 production after phacoemulsifica-tion.

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