Elevated expression of PDI family proteins during differentiation of mouse F9 teratocarcinoma cells

Osamu Miyaishi, Ken Ichi Kozaki, Ken Ichi Iida, Ken Ichi Isobe, Yoshio Hashizume, Shinsuke Saga

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We investigated the expression of protein disulfide isomerase family proteins (PDI, ERp61, and ERp72) in mouse F9 teratocarcinoma cells during differentiation induced by treatment with retinoic acid and dibutyryl cAMP. Each member of this family was expressed at a constitutive level in undifferentiated F9 cells. During differentiation of F9 cells to parietal or visceral endodermal cells the protein level of all these enzymes increased, although the extent of this increase in both protein and mRNA levels varied among the enzymes. Certain proteins were found to be coimmunoprecipitated with PDI, ERp61, and ERp72 in the presence of a chemical crosslinker. Type IV collagen was significantly coprecipitated with PDI whereas laminin was equally coprecipitated with the three proteins. Furthermore, 210 kDa protein characteristically coprecipitated with ERp72. Thus, the induction of PDI family proteins during the differentiation of F9 cells and their association with different proteins may implicate specific functions of each member of this family despite the common redox activity capable of catalyzing the disulfide bond formation.

Original languageEnglish
Pages (from-to)436-445
Number of pages10
JournalJournal of Cellular Biochemistry
Volume68
Issue number4
DOIs
Publication statusPublished - Mar 15 1998
Externally publishedYes

Fingerprint

Teratocarcinoma
Proteins
Cell Differentiation
Protein Disulfide-Isomerases
Collagen Type IV
Laminin
Enzymes
Tretinoin
Disulfides
Oxidation-Reduction
Messenger RNA

Keywords

  • Dibutyryl cAMP
  • Differentiation
  • Mouse
  • PDI family proteins
  • Retinoic acid

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Elevated expression of PDI family proteins during differentiation of mouse F9 teratocarcinoma cells. / Miyaishi, Osamu; Kozaki, Ken Ichi; Iida, Ken Ichi; Isobe, Ken Ichi; Hashizume, Yoshio; Saga, Shinsuke.

In: Journal of Cellular Biochemistry, Vol. 68, No. 4, 15.03.1998, p. 436-445.

Research output: Contribution to journalArticle

Miyaishi, Osamu ; Kozaki, Ken Ichi ; Iida, Ken Ichi ; Isobe, Ken Ichi ; Hashizume, Yoshio ; Saga, Shinsuke. / Elevated expression of PDI family proteins during differentiation of mouse F9 teratocarcinoma cells. In: Journal of Cellular Biochemistry. 1998 ; Vol. 68, No. 4. pp. 436-445.
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