Elevated expression of caveolin is associated with prostate and breast cancer

Guang Yang, Luan D. Truong, Terry L. Timme, Chengzhen Ren, Thomas M. Wheeler, Sang Hee Park, Yasutomo Nasu, Chris H. Bangma, Michael W. Kattan, Peter T. Scardino, Timothy C. Thompson

Research output: Contribution to journalArticle

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Abstract

To identify genes associated with prostate cancer progression, we developed a strategy involving the use of differential display-PCR with a panel of genetically matched primary tumor- and metastasis-derived mouse prostate cancer cell lines. We isolated a cDNA fragment with homology to the mouse caveolin-1 gene. Northern blotting with this fragment revealed increased caveolin expression in metastasis-derived cell lines relative to primary tumor-derived cell lines. Western blotting with a polyclonal caveolin antibody confirmed increased caveolin protein in metastasis-derived mouse cell lines and expression in three of four human prostate cancer cell lines. Immunohistochemical analysis of a human prostate cancer cell line demonstrated a prominent granular pattern of caveolin accumulation. Subsequent analysis of mouse and human prostate specimens revealed minimal caveolin expression in normal epithelium with abundant staining of smooth muscle and endothelium. The frequency of caveolin-positive cells was increased in prostate cancer with markedly increased accumulation of caveolin and a granular staining pattern in lymph node metastatic deposits. In human breast cancer specimens, increased caveolin staining was detected in intraductal and infiltrating ductal carcinoma as well as nodal disease. Caveolin therefore appears to be associated with human prostate cancer progression and is also present in primary and metastatic human breast cancer.

Original languageEnglish
Pages (from-to)1873-1880
Number of pages8
JournalClinical Cancer Research
Volume4
Issue number8
Publication statusPublished - Aug 1998
Externally publishedYes

Fingerprint

Caveolins
Prostatic Neoplasms
Breast Neoplasms
Cell Line
Staining and Labeling
Neoplasm Metastasis
Caveolin 1
Ductal Carcinoma
Tumor Cell Line
Northern Blotting
Genes
Endothelium
Smooth Muscle
Prostate
Epithelium
Complementary DNA
Lymph Nodes
Western Blotting
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Yang, G., Truong, L. D., Timme, T. L., Ren, C., Wheeler, T. M., Park, S. H., ... Thompson, T. C. (1998). Elevated expression of caveolin is associated with prostate and breast cancer. Clinical Cancer Research, 4(8), 1873-1880.

Elevated expression of caveolin is associated with prostate and breast cancer. / Yang, Guang; Truong, Luan D.; Timme, Terry L.; Ren, Chengzhen; Wheeler, Thomas M.; Park, Sang Hee; Nasu, Yasutomo; Bangma, Chris H.; Kattan, Michael W.; Scardino, Peter T.; Thompson, Timothy C.

In: Clinical Cancer Research, Vol. 4, No. 8, 08.1998, p. 1873-1880.

Research output: Contribution to journalArticle

Yang, G, Truong, LD, Timme, TL, Ren, C, Wheeler, TM, Park, SH, Nasu, Y, Bangma, CH, Kattan, MW, Scardino, PT & Thompson, TC 1998, 'Elevated expression of caveolin is associated with prostate and breast cancer', Clinical Cancer Research, vol. 4, no. 8, pp. 1873-1880.
Yang G, Truong LD, Timme TL, Ren C, Wheeler TM, Park SH et al. Elevated expression of caveolin is associated with prostate and breast cancer. Clinical Cancer Research. 1998 Aug;4(8):1873-1880.
Yang, Guang ; Truong, Luan D. ; Timme, Terry L. ; Ren, Chengzhen ; Wheeler, Thomas M. ; Park, Sang Hee ; Nasu, Yasutomo ; Bangma, Chris H. ; Kattan, Michael W. ; Scardino, Peter T. ; Thompson, Timothy C. / Elevated expression of caveolin is associated with prostate and breast cancer. In: Clinical Cancer Research. 1998 ; Vol. 4, No. 8. pp. 1873-1880.
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