Electrostatic interaction of positive charges on the surface of Psb31 with photosystem II in the diatom Chaetoceros gracilis

Ryo Nagao, Takehiro Suzuki, Akinori Okumura, Tomohiro Kihira, Ayaka Toda, Naoshi Dohmae, Katsuyoshi Nakazato, Tatsuya Tomo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Psb31, a novel extrinsic protein found in diatom photosystem II (PSII), directly binds to PSII core subunits, independent of the other extrinsic proteins, and functions to maintain optimum oxygen evolution. However, how Psb31 electrostatically interacts with PSII intrinsic proteins remains to be clarified. In this study, we examined electrostatic interaction of Psb31 with PSII complexes isolated from the diatom Chaetoceros gracilis. Positive or negative charges of isolated Psb31 proteins were modified with N-succinimidyl propionate (NSP) or glycine methyl ester (GME), respectively, resulting in formation of uncharged groups. NSP-modified Psb31 did not bind to PSII with a concomitant increase in NSP concentration, whereas GME-modified Psb31 clearly bound to PSII with retention of oxygen-evolving activity, indicating that positive charges of Lys residues and the N-terminus on the surface of Psb31 are involved in electrostatic interactions with PSII intrinsic proteins. Mass spectrometry analysis of NSP-modified Psb31 and sequence comparisons of Psb31 from C. gracilis with other chromophyte algae led to identification of three Lys residues as possible binding sites to PSII. Based on these findings, together with our previous cross-linking study in diatom PSII and a red algal PSII structure, we discuss binding properties of Psb31 with PSII core proteins.

Original languageEnglish
Pages (from-to)779-785
Number of pages7
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1858
Issue number9
DOIs
Publication statusPublished - Sep 1 2017
Externally publishedYes

Fingerprint

Diatoms
Photosystem II Protein Complex
Coulomb interactions
Static Electricity
Proteins
Oxygen
Algae
Mass spectrometry
Mass Spectrometry
Binding Sites

Keywords

  • Diatom
  • Extrinsic proteins
  • Photosystem II
  • Psb31

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

Electrostatic interaction of positive charges on the surface of Psb31 with photosystem II in the diatom Chaetoceros gracilis. / Nagao, Ryo; Suzuki, Takehiro; Okumura, Akinori; Kihira, Tomohiro; Toda, Ayaka; Dohmae, Naoshi; Nakazato, Katsuyoshi; Tomo, Tatsuya.

In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 1858, No. 9, 01.09.2017, p. 779-785.

Research output: Contribution to journalArticle

Nagao, Ryo ; Suzuki, Takehiro ; Okumura, Akinori ; Kihira, Tomohiro ; Toda, Ayaka ; Dohmae, Naoshi ; Nakazato, Katsuyoshi ; Tomo, Tatsuya. / Electrostatic interaction of positive charges on the surface of Psb31 with photosystem II in the diatom Chaetoceros gracilis. In: Biochimica et Biophysica Acta - Bioenergetics. 2017 ; Vol. 1858, No. 9. pp. 779-785.
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