Electrophysiological properties of the rat area postrema neurons displaying both the transient outward current and the hyperpolarization-activated inward current

Makoto Funahashi, Yoshihiro Mitoh, Ryuji Matsuo

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10 Citations (Scopus)

Abstract

We found coexistence of the transient outward potassium current (ITO) and the hyperpolarization-activated inward current (IH) in 26 of 82 area postrema neurons tested using the whole-cell patch-clamp technique in rat brain slices. Cells displaying both the ITO and the IH typically showed "voltage sag" and "rebound potentials" in response to hyperpolarizing current injection and repetitive firing with strong adaptation was seen with depolarizing current injection. When cells were held at membrane potentials more negative than the resting level (e.g., -85mV), the afterhyperpolarization was enhanced. Voltage clamp recordings were performed to examine the characteristics of ITO and IH in and the contributions of these currents to the electroresponsiveness of area postrema cells. We show, in this study, the voltage-dependent properties of IH and ITO, and how these currents modulate the intrinsic membrane properties of area postrema cells. We discuss the functional significance of the specific subset of area postrema neurons whose cells have both IH and ITO channels.

Original languageEnglish
Pages (from-to)337-343
Number of pages7
JournalBrain Research Bulletin
Volume58
Issue number3
DOIs
Publication statusPublished - 2002

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Area Postrema
Neurons
Injections
Patch-Clamp Techniques
Membrane Potentials
Potassium
Membranes
Brain

Keywords

  • Area postrema
  • Hyperpolarization-activated inward current
  • Rat
  • Slice
  • Transient outward current
  • Whole-cell patch-clamp

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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abstract = "We found coexistence of the transient outward potassium current (ITO) and the hyperpolarization-activated inward current (IH) in 26 of 82 area postrema neurons tested using the whole-cell patch-clamp technique in rat brain slices. Cells displaying both the ITO and the IH typically showed {"}voltage sag{"} and {"}rebound potentials{"} in response to hyperpolarizing current injection and repetitive firing with strong adaptation was seen with depolarizing current injection. When cells were held at membrane potentials more negative than the resting level (e.g., -85mV), the afterhyperpolarization was enhanced. Voltage clamp recordings were performed to examine the characteristics of ITO and IH in and the contributions of these currents to the electroresponsiveness of area postrema cells. We show, in this study, the voltage-dependent properties of IH and ITO, and how these currents modulate the intrinsic membrane properties of area postrema cells. We discuss the functional significance of the specific subset of area postrema neurons whose cells have both IH and ITO channels.",
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AU - Funahashi, Makoto

AU - Mitoh, Yoshihiro

AU - Matsuo, Ryuji

PY - 2002

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N2 - We found coexistence of the transient outward potassium current (ITO) and the hyperpolarization-activated inward current (IH) in 26 of 82 area postrema neurons tested using the whole-cell patch-clamp technique in rat brain slices. Cells displaying both the ITO and the IH typically showed "voltage sag" and "rebound potentials" in response to hyperpolarizing current injection and repetitive firing with strong adaptation was seen with depolarizing current injection. When cells were held at membrane potentials more negative than the resting level (e.g., -85mV), the afterhyperpolarization was enhanced. Voltage clamp recordings were performed to examine the characteristics of ITO and IH in and the contributions of these currents to the electroresponsiveness of area postrema cells. We show, in this study, the voltage-dependent properties of IH and ITO, and how these currents modulate the intrinsic membrane properties of area postrema cells. We discuss the functional significance of the specific subset of area postrema neurons whose cells have both IH and ITO channels.

AB - We found coexistence of the transient outward potassium current (ITO) and the hyperpolarization-activated inward current (IH) in 26 of 82 area postrema neurons tested using the whole-cell patch-clamp technique in rat brain slices. Cells displaying both the ITO and the IH typically showed "voltage sag" and "rebound potentials" in response to hyperpolarizing current injection and repetitive firing with strong adaptation was seen with depolarizing current injection. When cells were held at membrane potentials more negative than the resting level (e.g., -85mV), the afterhyperpolarization was enhanced. Voltage clamp recordings were performed to examine the characteristics of ITO and IH in and the contributions of these currents to the electroresponsiveness of area postrema cells. We show, in this study, the voltage-dependent properties of IH and ITO, and how these currents modulate the intrinsic membrane properties of area postrema cells. We discuss the functional significance of the specific subset of area postrema neurons whose cells have both IH and ITO channels.

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