Electrophoretic and serologic characterization of 56 kDa antigen (M56) with autologous serum derived from a chondrosarcoma patient: A shared antigen of immunoresponses in cancer and autoimmune diseases

Kazuo Fujiwara, Heiichiro Udono, Toshiyuki Kunisada, Akira Kawai, Hajime Inoue, Masaharu Takigawa, Masayoshi Namba, Eiichi Nakayama

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Abstract

We investigated whether antibodies specific to autologous cancer cells are produced in the peripheral blood of patients with chondrosarcoma. There have been few reports on the investigation of the immune responses, such as autologous antibody production, to chondrosarcoma. Here, tumor-associated antigens were separated by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis and detected by immunoenzymatic amplification. A 56 kDa molecule (M56) was detected in the serum from patients' peripheral blood. M56 is ubiquitously expressed in various kinds of tissue-derived cells. However, the molecule seemed to be retained mostly in the cytosolic compartment of lymphoid cells, while it was expressed on the cell surface of nonlymphoid cancer cells. Furthermore, the antibodies reactive to the 56 kDa molecule were frequently observed in sera derived from patients with other cancers and autoimmune diseases as compared to the sera from healthy control donors, suggesting that M56 is a common target molecule of immune responses in patients with various cancers and autoimmune diseases.

Original languageEnglish
Pages (from-to)3335-3342
Number of pages8
JournalElectrophoresis
Volume20
Issue number17
Publication statusPublished - 1999

Fingerprint

Chondrosarcoma
Autoimmune Diseases
Antigens
Molecules
Serum
Antibodies
Neoplasms
Blood
Cells
Neoplasm Antigens
Electrophoresis
Sodium Dodecyl Sulfate
Antibody Formation
Amplification
Tumors
Polyacrylamide Gel Electrophoresis
Tissue Donors
Lymphocytes
Tissue

Keywords

  • Autoantibody
  • Autoimmune diseases
  • Chondrosarcoma
  • Shared antigen
  • Western blotting

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

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title = "Electrophoretic and serologic characterization of 56 kDa antigen (M56) with autologous serum derived from a chondrosarcoma patient: A shared antigen of immunoresponses in cancer and autoimmune diseases",
abstract = "We investigated whether antibodies specific to autologous cancer cells are produced in the peripheral blood of patients with chondrosarcoma. There have been few reports on the investigation of the immune responses, such as autologous antibody production, to chondrosarcoma. Here, tumor-associated antigens were separated by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis and detected by immunoenzymatic amplification. A 56 kDa molecule (M56) was detected in the serum from patients' peripheral blood. M56 is ubiquitously expressed in various kinds of tissue-derived cells. However, the molecule seemed to be retained mostly in the cytosolic compartment of lymphoid cells, while it was expressed on the cell surface of nonlymphoid cancer cells. Furthermore, the antibodies reactive to the 56 kDa molecule were frequently observed in sera derived from patients with other cancers and autoimmune diseases as compared to the sera from healthy control donors, suggesting that M56 is a common target molecule of immune responses in patients with various cancers and autoimmune diseases.",
keywords = "Autoantibody, Autoimmune diseases, Chondrosarcoma, Shared antigen, Western blotting",
author = "Kazuo Fujiwara and Heiichiro Udono and Toshiyuki Kunisada and Akira Kawai and Hajime Inoue and Masaharu Takigawa and Masayoshi Namba and Eiichi Nakayama",
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T2 - A shared antigen of immunoresponses in cancer and autoimmune diseases

AU - Fujiwara, Kazuo

AU - Udono, Heiichiro

AU - Kunisada, Toshiyuki

AU - Kawai, Akira

AU - Inoue, Hajime

AU - Takigawa, Masaharu

AU - Namba, Masayoshi

AU - Nakayama, Eiichi

PY - 1999

Y1 - 1999

N2 - We investigated whether antibodies specific to autologous cancer cells are produced in the peripheral blood of patients with chondrosarcoma. There have been few reports on the investigation of the immune responses, such as autologous antibody production, to chondrosarcoma. Here, tumor-associated antigens were separated by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis and detected by immunoenzymatic amplification. A 56 kDa molecule (M56) was detected in the serum from patients' peripheral blood. M56 is ubiquitously expressed in various kinds of tissue-derived cells. However, the molecule seemed to be retained mostly in the cytosolic compartment of lymphoid cells, while it was expressed on the cell surface of nonlymphoid cancer cells. Furthermore, the antibodies reactive to the 56 kDa molecule were frequently observed in sera derived from patients with other cancers and autoimmune diseases as compared to the sera from healthy control donors, suggesting that M56 is a common target molecule of immune responses in patients with various cancers and autoimmune diseases.

AB - We investigated whether antibodies specific to autologous cancer cells are produced in the peripheral blood of patients with chondrosarcoma. There have been few reports on the investigation of the immune responses, such as autologous antibody production, to chondrosarcoma. Here, tumor-associated antigens were separated by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis and detected by immunoenzymatic amplification. A 56 kDa molecule (M56) was detected in the serum from patients' peripheral blood. M56 is ubiquitously expressed in various kinds of tissue-derived cells. However, the molecule seemed to be retained mostly in the cytosolic compartment of lymphoid cells, while it was expressed on the cell surface of nonlymphoid cancer cells. Furthermore, the antibodies reactive to the 56 kDa molecule were frequently observed in sera derived from patients with other cancers and autoimmune diseases as compared to the sera from healthy control donors, suggesting that M56 is a common target molecule of immune responses in patients with various cancers and autoimmune diseases.

KW - Autoantibody

KW - Autoimmune diseases

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KW - Shared antigen

KW - Western blotting

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