Electronic structure of S2 state of the oxygen-evolving complex of photosystem II studied by PELDOR

Mizue Asada, Hiroki Nagashima, Faisal Hammad Mekky Koua, Jian-Ren Shen, Asako Kawamori, Hiroyuki Mino

Research output: Contribution to journalArticle

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Abstract

Photosynthetic water splitting is catalyzed by a Mn4CaO 5 cluster in photosystem II, whose structure was recently determined at a resolution of 1.9 Å [Umena, Y. et al. 2011, Nature, 473:55-60]. To determine the electronic structure of the Mn4CaO5 cluster, pulsed electron-electron double resonance (PELDOR) measurements were performed for the tyrosine residue YD and S2 state signals with non-oriented and oriented photosystem II (PS II) samples. Based on these measurements, the spin density distributions were calculated by comparing with the experimental results. The best fitting parameters were obtained with a model in which Mn1 has a large positive projection, Mn3 has a small positive projection, and Mn2 and Mn4 have negative projections (the numbering of Mni (i = 1-4) is based on the crystal structure at a 1.9 Å resolution), which yielded spin projections of 1.97, - 1.20, 1.19 and - 0.96 for Mn1-4 ions. The results show that the Mn1 ion, which is coordinated by H332, D342 and E189, has a valence of Mn(III) in the S2 state. The sign of the exchange interactions J13 is positive, and the other signs are negative.

Original languageEnglish
Pages (from-to)438-445
Number of pages8
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1827
Issue number3
DOIs
Publication statusPublished - Mar 2013

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Photosystem II Protein Complex
Electronic structure
Electrons
Ions
Oxygen
Exchange interactions
Tyrosine
Crystal structure
Water

Keywords

  • EPR
  • Mn cluster
  • Oxygen-evolving complex
  • PELDOR
  • Photosystem II

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

Electronic structure of S2 state of the oxygen-evolving complex of photosystem II studied by PELDOR. / Asada, Mizue; Nagashima, Hiroki; Koua, Faisal Hammad Mekky; Shen, Jian-Ren; Kawamori, Asako; Mino, Hiroyuki.

In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 1827, No. 3, 03.2013, p. 438-445.

Research output: Contribution to journalArticle

Asada, Mizue ; Nagashima, Hiroki ; Koua, Faisal Hammad Mekky ; Shen, Jian-Ren ; Kawamori, Asako ; Mino, Hiroyuki. / Electronic structure of S2 state of the oxygen-evolving complex of photosystem II studied by PELDOR. In: Biochimica et Biophysica Acta - Bioenergetics. 2013 ; Vol. 1827, No. 3. pp. 438-445.
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