Electron spin resonance spin trapping assay and immunohistochemical localization of superoxide dismutases in the rat nasal mucosa

Ming Tang Lai, Takuya Ohmichi, Teruhiro Ogawa, Kazunori Nishizaki, Yu Masuda

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The immunohistochemical method and electron spin resonance (ESR) spin trapping assay were employed to detect the localization and biochemical activity of superoxide dismutases (SODs) in the rat nasal mucosa. Manganese SOD and copper-zinc SOD were immunohistochemically illustrated to be richly expressed in the epithelial cells and the subepithelial glands of nasal mucosa. The olfactory vesicles also showed positive immunostaining for manganese SOD and copper-zinc SOD. ESR spin trapping assay revealed that SOD activity in the mucosa of olfactory areas was significantly higher than in the mucosa of respiratory areas; however, the ratio of SOD activity in the mitochondrial fraction to SOD activity in the cytosolic fraction was similar, approximating 17: 83 in the mucosa of both the olfactory and respiratory areas. The predominant localization of SODs in epithelial cells of nasal mucosa suggests the importance of mucosal epithelium in protecting nasal mucosa against cytotoxic superoxide (O2) radicals. Epithelial goblet cells and the connective tissue of lamina propria, which showed no positive immunostaining for SODs, are considered to be vulnerable to oxidative insults implicated in the generation of O2 radicals. The higher SODs activity in the mucosa of olfactory areas implies that there is a different requirement of SOD in mucosa of the respiratory and olfactory areas on scavenging microenvironmental O2 radicals.

Original languageEnglish
Pages (from-to)437-446
Number of pages10
JournalActa Oto-Laryngologica
Volume117
Issue number3
Publication statusPublished - 1997

Fingerprint

Spin Trapping
Nasal Mucosa
Electron Spin Resonance Spectroscopy
Superoxide Dismutase
Olfactory Mucosa
Respiratory Mucosa
Epithelial Cells
Zinc
Copper
Goblet Cells
Superoxides
Connective Tissue
Mucous Membrane

Keywords

  • electron spin resonance
  • immunohistochemistry
  • olfactory mucosa
  • respiratory mucosa
  • superoxide

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Electron spin resonance spin trapping assay and immunohistochemical localization of superoxide dismutases in the rat nasal mucosa. / Lai, Ming Tang; Ohmichi, Takuya; Ogawa, Teruhiro; Nishizaki, Kazunori; Masuda, Yu.

In: Acta Oto-Laryngologica, Vol. 117, No. 3, 1997, p. 437-446.

Research output: Contribution to journalArticle

@article{33cee0c7bd1a40fd94eb6f739bc177cb,
title = "Electron spin resonance spin trapping assay and immunohistochemical localization of superoxide dismutases in the rat nasal mucosa",
abstract = "The immunohistochemical method and electron spin resonance (ESR) spin trapping assay were employed to detect the localization and biochemical activity of superoxide dismutases (SODs) in the rat nasal mucosa. Manganese SOD and copper-zinc SOD were immunohistochemically illustrated to be richly expressed in the epithelial cells and the subepithelial glands of nasal mucosa. The olfactory vesicles also showed positive immunostaining for manganese SOD and copper-zinc SOD. ESR spin trapping assay revealed that SOD activity in the mucosa of olfactory areas was significantly higher than in the mucosa of respiratory areas; however, the ratio of SOD activity in the mitochondrial fraction to SOD activity in the cytosolic fraction was similar, approximating 17: 83 in the mucosa of both the olfactory and respiratory areas. The predominant localization of SODs in epithelial cells of nasal mucosa suggests the importance of mucosal epithelium in protecting nasal mucosa against cytotoxic superoxide (O2) radicals. Epithelial goblet cells and the connective tissue of lamina propria, which showed no positive immunostaining for SODs, are considered to be vulnerable to oxidative insults implicated in the generation of O2 radicals. The higher SODs activity in the mucosa of olfactory areas implies that there is a different requirement of SOD in mucosa of the respiratory and olfactory areas on scavenging microenvironmental O2 radicals.",
keywords = "electron spin resonance, immunohistochemistry, olfactory mucosa, respiratory mucosa, superoxide",
author = "Lai, {Ming Tang} and Takuya Ohmichi and Teruhiro Ogawa and Kazunori Nishizaki and Yu Masuda",
year = "1997",
language = "English",
volume = "117",
pages = "437--446",
journal = "Acta Oto-Laryngologica",
issn = "0001-6489",
publisher = "Informa Healthcare",
number = "3",

}

TY - JOUR

T1 - Electron spin resonance spin trapping assay and immunohistochemical localization of superoxide dismutases in the rat nasal mucosa

AU - Lai, Ming Tang

AU - Ohmichi, Takuya

AU - Ogawa, Teruhiro

AU - Nishizaki, Kazunori

AU - Masuda, Yu

PY - 1997

Y1 - 1997

N2 - The immunohistochemical method and electron spin resonance (ESR) spin trapping assay were employed to detect the localization and biochemical activity of superoxide dismutases (SODs) in the rat nasal mucosa. Manganese SOD and copper-zinc SOD were immunohistochemically illustrated to be richly expressed in the epithelial cells and the subepithelial glands of nasal mucosa. The olfactory vesicles also showed positive immunostaining for manganese SOD and copper-zinc SOD. ESR spin trapping assay revealed that SOD activity in the mucosa of olfactory areas was significantly higher than in the mucosa of respiratory areas; however, the ratio of SOD activity in the mitochondrial fraction to SOD activity in the cytosolic fraction was similar, approximating 17: 83 in the mucosa of both the olfactory and respiratory areas. The predominant localization of SODs in epithelial cells of nasal mucosa suggests the importance of mucosal epithelium in protecting nasal mucosa against cytotoxic superoxide (O2) radicals. Epithelial goblet cells and the connective tissue of lamina propria, which showed no positive immunostaining for SODs, are considered to be vulnerable to oxidative insults implicated in the generation of O2 radicals. The higher SODs activity in the mucosa of olfactory areas implies that there is a different requirement of SOD in mucosa of the respiratory and olfactory areas on scavenging microenvironmental O2 radicals.

AB - The immunohistochemical method and electron spin resonance (ESR) spin trapping assay were employed to detect the localization and biochemical activity of superoxide dismutases (SODs) in the rat nasal mucosa. Manganese SOD and copper-zinc SOD were immunohistochemically illustrated to be richly expressed in the epithelial cells and the subepithelial glands of nasal mucosa. The olfactory vesicles also showed positive immunostaining for manganese SOD and copper-zinc SOD. ESR spin trapping assay revealed that SOD activity in the mucosa of olfactory areas was significantly higher than in the mucosa of respiratory areas; however, the ratio of SOD activity in the mitochondrial fraction to SOD activity in the cytosolic fraction was similar, approximating 17: 83 in the mucosa of both the olfactory and respiratory areas. The predominant localization of SODs in epithelial cells of nasal mucosa suggests the importance of mucosal epithelium in protecting nasal mucosa against cytotoxic superoxide (O2) radicals. Epithelial goblet cells and the connective tissue of lamina propria, which showed no positive immunostaining for SODs, are considered to be vulnerable to oxidative insults implicated in the generation of O2 radicals. The higher SODs activity in the mucosa of olfactory areas implies that there is a different requirement of SOD in mucosa of the respiratory and olfactory areas on scavenging microenvironmental O2 radicals.

KW - electron spin resonance

KW - immunohistochemistry

KW - olfactory mucosa

KW - respiratory mucosa

KW - superoxide

UR - http://www.scopus.com/inward/record.url?scp=0030904533&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030904533&partnerID=8YFLogxK

M3 - Article

C2 - 9199532

AN - SCOPUS:0030904533

VL - 117

SP - 437

EP - 446

JO - Acta Oto-Laryngologica

JF - Acta Oto-Laryngologica

SN - 0001-6489

IS - 3

ER -