Electron microscopic observations of aberrant capsids of pseudorabies virus

Yuko Muraki, Masao Yamada, Mariko Yoshida, Shunji Yamada, Jorge Padilla, Yoshihiro Hatano, Yoshio Hiramatsu, Fumio Uno, Shiro Nii

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Interactions between four strains of pseudorabies virus (PrV) and seven kinds of cell lines were examined. Three kinds of cells (SKL, CPK and PK-15) were especially infected with PrV at an MOI of about 10 PFU/cell. At sequential intervals after infection, cells of these types were collected for electron microscopic observations and the infectious doses of culture fluids were assayed. Developmental features of PrV were found to be very similar to those of herpes simplex virus (HSV), with the slight difference that PrV developed a little earlier and more vigorously than did HSV. At the late stage of infection, aberrant capsids of PrV were observed frequently in the nucleus of SKL but rarely in the nuclei of CPK and PK-15. The titer of infectious virus produced by SKL was much larger than that produced by CPK and PK-15. Immuno-electron microscopic examination using a monoclonal antibody against the major capsid protein of PrV clearly demonstrated that the complete and aberrant capsids have a common epitope. The mechanism of the formation of aberrant capsids is discussed.

Original languageEnglish
Pages (from-to)223-231
Number of pages9
JournalJournal of Electron Microscopy
Volume45
Issue number3
Publication statusPublished - 1996

Fingerprint

Suid Herpesvirus 1
Capsid
viruses
Viruses
Electrons
electrons
Simplexvirus
infectious diseases
Capsid Proteins
Infection
Viral Load
cells
Epitopes
nuclei
Monoclonal antibodies
Monoclonal Antibodies
antibodies
cultured cells
Cell Line
Microscopic examination

Keywords

  • Aberrant capsid
  • Herpesvirus
  • Immuno-electron microscopy
  • Pseudorabies virus
  • Virus morphology

ASJC Scopus subject areas

  • Instrumentation

Cite this

Muraki, Y., Yamada, M., Yoshida, M., Yamada, S., Padilla, J., Hatano, Y., ... Nii, S. (1996). Electron microscopic observations of aberrant capsids of pseudorabies virus. Journal of Electron Microscopy, 45(3), 223-231.

Electron microscopic observations of aberrant capsids of pseudorabies virus. / Muraki, Yuko; Yamada, Masao; Yoshida, Mariko; Yamada, Shunji; Padilla, Jorge; Hatano, Yoshihiro; Hiramatsu, Yoshio; Uno, Fumio; Nii, Shiro.

In: Journal of Electron Microscopy, Vol. 45, No. 3, 1996, p. 223-231.

Research output: Contribution to journalArticle

Muraki, Y, Yamada, M, Yoshida, M, Yamada, S, Padilla, J, Hatano, Y, Hiramatsu, Y, Uno, F & Nii, S 1996, 'Electron microscopic observations of aberrant capsids of pseudorabies virus', Journal of Electron Microscopy, vol. 45, no. 3, pp. 223-231.
Muraki Y, Yamada M, Yoshida M, Yamada S, Padilla J, Hatano Y et al. Electron microscopic observations of aberrant capsids of pseudorabies virus. Journal of Electron Microscopy. 1996;45(3):223-231.
Muraki, Yuko ; Yamada, Masao ; Yoshida, Mariko ; Yamada, Shunji ; Padilla, Jorge ; Hatano, Yoshihiro ; Hiramatsu, Yoshio ; Uno, Fumio ; Nii, Shiro. / Electron microscopic observations of aberrant capsids of pseudorabies virus. In: Journal of Electron Microscopy. 1996 ; Vol. 45, No. 3. pp. 223-231.
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