Electron microscopic demonstration of nick end-labeled DNA fragments during capsaicin-induced apoptosis of trigeminal primary neurons in neonatal rats

Tomosada Sugimoto, Akihiro Takeyama, Chun Xiao, Teruko Takano-Yamamoto, Hiroyuki Ichikawa

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

DNA fragmentation was induced in the trigeminal ganglion of newborn rats by subcutaneous capsaicin injection (50 mg/kg). Twenty-four hours later, numerous roundish profiles were intensely labeled by both a DNA polymerase I- mediated nick translation method and a terminal transferase-mediated tailing method. Direct electron microscopic examination of labeled profiles indicated that the labeled profiles were neurons at earlier stages of apoptosis. DNA fragmentation signal was first detected in the nucleoplasm and later spread to the cytoplasm. The cell finally disintegrated forming many small apoptotic bodies. DNA fragmentation signal in the apoptotic bodies was readily labeled by the tailing but not the translation method.

Original languageEnglish
Pages (from-to)147-152
Number of pages6
JournalBrain Research
Volume818
Issue number1
DOIs
Publication statusPublished - Feb 6 1999

Fingerprint

Capsaicin
DNA Fragmentation
Electrons
Apoptosis
Neurons
DNA
Trigeminal Ganglion
DNA Polymerase I
Subcutaneous Injections
Transferases
Cytoplasm
Extracellular Vesicles

Keywords

  • Apoptosis
  • Capsaicin
  • Electron microscopy
  • Primary sensory neuron
  • Trigeminal ganglion
  • TUNEL

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Electron microscopic demonstration of nick end-labeled DNA fragments during capsaicin-induced apoptosis of trigeminal primary neurons in neonatal rats. / Sugimoto, Tomosada; Takeyama, Akihiro; Xiao, Chun; Takano-Yamamoto, Teruko; Ichikawa, Hiroyuki.

In: Brain Research, Vol. 818, No. 1, 06.02.1999, p. 147-152.

Research output: Contribution to journalArticle

Sugimoto, Tomosada ; Takeyama, Akihiro ; Xiao, Chun ; Takano-Yamamoto, Teruko ; Ichikawa, Hiroyuki. / Electron microscopic demonstration of nick end-labeled DNA fragments during capsaicin-induced apoptosis of trigeminal primary neurons in neonatal rats. In: Brain Research. 1999 ; Vol. 818, No. 1. pp. 147-152.
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