Electron microscopic autoradiographic study on immune complex formation in rat glomerulus with anti-heparan sulfate proteoglycan antibody

Hirofumi Makino, Naoki Kashihara, Zensuke Ota, Yashpal S. Kanwar

Research output: Contribution to journalArticle

Abstract

Using electron microscopic autoradiography, we studied the mechanism of the formation of immune complex in the subepithelial aspect of glomerular basement membrane in rats, as a model for glomerulonephritis in man. Rats were immunized with normal rabbit IgG, and were administered intravenously with radioiodinated rabbit anti-heparan sulfate proteoglycan (HS-PG) antibody. Two weeks later the center of the grains appeared to correspond with the center of the subepithelial electron dense deposits, thus suggesting a topological superimposition of the radioiodinated anti-HS-PG antibody over the immune complex deposits located in the subepithelial space. From our present study it has become clear that the HS-PG site may serve as the corenidus for the formation of immune complex deposit. Electron microscopic autoradiography is very helpful in the demonstrating of in situ immune complex formation in glomerulonephritis.

Original languageEnglish
Pages (from-to)358-359
Number of pages2
JournalMedical Electron Microscopy
Volume27
Issue number3-4
DOIs
Publication statusPublished - Dec 1 1994

Keywords

  • Autoradiography
  • Glomerulonephritis
  • Heparan sulfate proteoglycan
  • immune complex

ASJC Scopus subject areas

  • Anatomy

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