Abstract
Using electron microscopic autoradiography, we studied the mechanism of the formation of immune complex in the subepithelial aspect of glomerular basement membrane in rats, as a model for glomerulonephritis in man. Rats were immunized with normal rabbit IgG, and were administered intravenously with radioiodinated rabbit anti-heparan sulfate proteoglycan (HS-PG) antibody. Two weeks later the center of the grains appeared to correspond with the center of the subepithelial electron dense deposits, thus suggesting a topological superimposition of the radioiodinated anti-HS-PG antibody over the immune complex deposits located in the subepithelial space. From our present study it has become clear that the HS-PG site may serve as the corenidus for the formation of immune complex deposit. Electron microscopic autoradiography is very helpful in the demonstrating of in situ immune complex formation in glomerulonephritis.
Original language | English |
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Pages (from-to) | 358-359 |
Number of pages | 2 |
Journal | Medical Electron Microscopy |
Volume | 27 |
Issue number | 3-4 |
DOIs | |
Publication status | Published - Dec 1 1994 |
Keywords
- Autoradiography
- Glomerulonephritis
- Heparan sulfate proteoglycan
- immune complex
ASJC Scopus subject areas
- Anatomy