Abstract
Using electron microscopic autoradiography, we studied the mechanism of the formation of immune complex in the subepithelial aspect of glomerular basement membrane in rats, as a model for glomerulonephritis in man. Rats were immunized with normal rabbit IgG, and were administered intravenously with radioiodinated rabbit anti-heparan sulfate proteoglycan (HS-PG) antibody. Two weeks later the center of the grains appeared to correspond with the center of the subepithelial electron dense deposits, thus suggesting a topological superimposition of the radioiodinated anti-HS-PG antibody over the immune complex deposits located in the subepithelial space. From our present study it has become clear that the HS-PG site may serve as the corenidus for the formation of immune complex deposit. Electron microscopic autoradiography is very helpful in the demonstrating of in situ immune complex formation in glomerulonephritis.
| Original language | English |
|---|---|
| Pages (from-to) | 358-359 |
| Number of pages | 2 |
| Journal | Medical Electron Microscopy |
| Volume | 27 |
| Issue number | 3-4 |
| DOIs | |
| Publication status | Published - Dec 1 1994 |
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Keywords
- Autoradiography
- Glomerulonephritis
- Heparan sulfate proteoglycan
- immune complex
ASJC Scopus subject areas
- Anatomy
Cite this
Electron microscopic autoradiographic study on immune complex formation in rat glomerulus with anti-heparan sulfate proteoglycan antibody. / Makino, Hirofumi; Kashihara, Naoki; Ota, Zensuke; Kanwar, Yashpal S.
In: Medical Electron Microscopy, Vol. 27, No. 3-4, 01.12.1994, p. 358-359.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Electron microscopic autoradiographic study on immune complex formation in rat glomerulus with anti-heparan sulfate proteoglycan antibody
AU - Makino, Hirofumi
AU - Kashihara, Naoki
AU - Ota, Zensuke
AU - Kanwar, Yashpal S.
PY - 1994/12/1
Y1 - 1994/12/1
N2 - Using electron microscopic autoradiography, we studied the mechanism of the formation of immune complex in the subepithelial aspect of glomerular basement membrane in rats, as a model for glomerulonephritis in man. Rats were immunized with normal rabbit IgG, and were administered intravenously with radioiodinated rabbit anti-heparan sulfate proteoglycan (HS-PG) antibody. Two weeks later the center of the grains appeared to correspond with the center of the subepithelial electron dense deposits, thus suggesting a topological superimposition of the radioiodinated anti-HS-PG antibody over the immune complex deposits located in the subepithelial space. From our present study it has become clear that the HS-PG site may serve as the corenidus for the formation of immune complex deposit. Electron microscopic autoradiography is very helpful in the demonstrating of in situ immune complex formation in glomerulonephritis.
AB - Using electron microscopic autoradiography, we studied the mechanism of the formation of immune complex in the subepithelial aspect of glomerular basement membrane in rats, as a model for glomerulonephritis in man. Rats were immunized with normal rabbit IgG, and were administered intravenously with radioiodinated rabbit anti-heparan sulfate proteoglycan (HS-PG) antibody. Two weeks later the center of the grains appeared to correspond with the center of the subepithelial electron dense deposits, thus suggesting a topological superimposition of the radioiodinated anti-HS-PG antibody over the immune complex deposits located in the subepithelial space. From our present study it has become clear that the HS-PG site may serve as the corenidus for the formation of immune complex deposit. Electron microscopic autoradiography is very helpful in the demonstrating of in situ immune complex formation in glomerulonephritis.
KW - Autoradiography
KW - Glomerulonephritis
KW - Heparan sulfate proteoglycan
KW - immune complex
UR - http://www.scopus.com/inward/record.url?scp=34249767674&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34249767674&partnerID=8YFLogxK
U2 - 10.1007/BF02349696
DO - 10.1007/BF02349696
M3 - Article
AN - SCOPUS:34249767674
VL - 27
SP - 358
EP - 359
JO - Medical Electron Microscopy
JF - Medical Electron Microscopy
SN - 0918-4287
IS - 3-4
ER -