Electrogenic reactions on the donor side of Mn-depleted photosystem II core particles in the presence of MnCl2 and synthetic trinuclear Mn-complexes

Vasily N. Kurashov, Suleyman Allakhverdiev, Sergey K. Zharmukhamedov, Toshi Nagata, Vyacheslav V. Klimov, Alexey Yu Semenov, Mahir D. Mamedov

Research output: Contribution to journalArticle

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Abstract

An electrometric technique was used to investigate the generation of a photovoltage (Δψ) by Mn-depleted spinach photosystem II (PS II) core particles incorporated into liposomes. In the presence of MnCl2, the fast kinetically unresolvable phase of Δψ generation, related to electron transfer between the redox-active tyrosine YZ and the primary plastoquinone acceptor QA was followed by an additional electrogenic phase (τ∼ 20 μs, ∼5% of the phase attributed to YZ oxQA -). The latter phase was ascribed to the transfer of an electron from the Mn, bound to the Mn-binding site of the PS II reaction center to the YZ ox. An additional electrogenicity observed upon addition of synthetic trinuclear Mn complex-1 has a τ∼ 50 μs (∼4% of the YZ oxQA) and τ∼ 160 ms (∼25%). The fast electrogenic component could be ascribed to reduction of YZ ox by Mn, delivered to the Mn-binding site in Mn-depleted samples after the release of the tripod ligands from the complex-1 while the slow electrogenic phase to the electron transfer from the Mn-containing complex-1 attached to the protein-water boundary to the oxidized Mn at the protein-embedded Mn-binding site.

Original languageEnglish
Pages (from-to)162-166
Number of pages5
JournalPhotochemical and Photobiological Sciences
Volume8
Issue number2
DOIs
Publication statusPublished - Feb 11 2009
Externally publishedYes

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Photosystem II Protein Complex
Binding Sites
Electrons
electron transfer
Plastoquinone
spinach
proteins
tripods
tyrosine
photovoltages
Liposomes
Tyrosine
Proteins
Ligands
ligands
Water
water
manganese chloride
electrons

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry

Cite this

Electrogenic reactions on the donor side of Mn-depleted photosystem II core particles in the presence of MnCl2 and synthetic trinuclear Mn-complexes. / Kurashov, Vasily N.; Allakhverdiev, Suleyman; Zharmukhamedov, Sergey K.; Nagata, Toshi; Klimov, Vyacheslav V.; Semenov, Alexey Yu; Mamedov, Mahir D.

In: Photochemical and Photobiological Sciences, Vol. 8, No. 2, 11.02.2009, p. 162-166.

Research output: Contribution to journalArticle

Kurashov, Vasily N. ; Allakhverdiev, Suleyman ; Zharmukhamedov, Sergey K. ; Nagata, Toshi ; Klimov, Vyacheslav V. ; Semenov, Alexey Yu ; Mamedov, Mahir D. / Electrogenic reactions on the donor side of Mn-depleted photosystem II core particles in the presence of MnCl2 and synthetic trinuclear Mn-complexes. In: Photochemical and Photobiological Sciences. 2009 ; Vol. 8, No. 2. pp. 162-166.
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