Efficient replication systems for hepatitis C virus using a new human hepatoma cell line

Nobuyuki Kato, Kyoko Mori, Ken ichi Abe, Hiromichi Dansako, Misao Kuroki, Yasuo Ariumi, Takaji Wakita, Masanori Ikeda

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Persistent hepatitis C virus (HCV) infection causes chronic liver diseases and is a serious global health problem. Cell culture-based persistent HCV RNA replication systems and infectious HCV production systems are widely used in HCV research. However, persistent HCV production systems have been developed only for HuH-7 hepatoma cells. Here we found a new human hepatoma cell line, Li23, that enables persistent HCV production and anti-HCV reagent assay. Li23's cDNA expression profile differed from HuH-7's, although the two cells had similar liver-specific expression profiles. We used HCV RNA with a specific combination of adaptive mutations to develop an HCV replicon system and genome-length HCV RNA replicating systems including a reporter assay system. Finally, Li23-derived cells persistently produced infectious virus of an HCV strain. Li23-derived cells are potentially useful for understanding the HCV life cycle and for finding antiviral targets.

Original languageEnglish
Pages (from-to)41-50
Number of pages10
JournalVirus research
Volume146
Issue number1-2
DOIs
Publication statusPublished - Jan 2009

Keywords

  • HCV RNA replication
  • Hepatitis C virus (HCV)
  • Infectious HCV production
  • Li23 cells
  • Reporter assay for anti-HCV reagents

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

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