TY - JOUR
T1 - Efficient replication systems for hepatitis C virus using a new human hepatoma cell line
AU - Kato, Nobuyuki
AU - Mori, Kyoko
AU - Abe, Ken ichi
AU - Dansako, Hiromichi
AU - Kuroki, Misao
AU - Ariumi, Yasuo
AU - Wakita, Takaji
AU - Ikeda, Masanori
N1 - Funding Information:
We thank Takashi Nakamura and Atsumi Morishita for their technical assistance. We also thank S. Hirohashi, M. Namba, and D. Trono for Li21, Li24, OUMS29, IHH10.3, and IHH12 cell lines. This work was supported by a grant-in-aid for research on hepatitis from the Ministry of Health, Labor and Welfare of Japan .
PY - 2009/1
Y1 - 2009/1
N2 - Persistent hepatitis C virus (HCV) infection causes chronic liver diseases and is a serious global health problem. Cell culture-based persistent HCV RNA replication systems and infectious HCV production systems are widely used in HCV research. However, persistent HCV production systems have been developed only for HuH-7 hepatoma cells. Here we found a new human hepatoma cell line, Li23, that enables persistent HCV production and anti-HCV reagent assay. Li23's cDNA expression profile differed from HuH-7's, although the two cells had similar liver-specific expression profiles. We used HCV RNA with a specific combination of adaptive mutations to develop an HCV replicon system and genome-length HCV RNA replicating systems including a reporter assay system. Finally, Li23-derived cells persistently produced infectious virus of an HCV strain. Li23-derived cells are potentially useful for understanding the HCV life cycle and for finding antiviral targets.
AB - Persistent hepatitis C virus (HCV) infection causes chronic liver diseases and is a serious global health problem. Cell culture-based persistent HCV RNA replication systems and infectious HCV production systems are widely used in HCV research. However, persistent HCV production systems have been developed only for HuH-7 hepatoma cells. Here we found a new human hepatoma cell line, Li23, that enables persistent HCV production and anti-HCV reagent assay. Li23's cDNA expression profile differed from HuH-7's, although the two cells had similar liver-specific expression profiles. We used HCV RNA with a specific combination of adaptive mutations to develop an HCV replicon system and genome-length HCV RNA replicating systems including a reporter assay system. Finally, Li23-derived cells persistently produced infectious virus of an HCV strain. Li23-derived cells are potentially useful for understanding the HCV life cycle and for finding antiviral targets.
KW - HCV RNA replication
KW - Hepatitis C virus (HCV)
KW - Infectious HCV production
KW - Li23 cells
KW - Reporter assay for anti-HCV reagents
UR - http://www.scopus.com/inward/record.url?scp=70350233422&partnerID=8YFLogxK
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U2 - 10.1016/j.virusres.2009.08.006
DO - 10.1016/j.virusres.2009.08.006
M3 - Article
C2 - 19720094
AN - SCOPUS:70350233422
VL - 146
SP - 41
EP - 50
JO - Virus Research
JF - Virus Research
SN - 0168-1702
IS - 1-2
ER -
