Abstract
Persistent hepatitis C virus (HCV) infection causes chronic liver diseases and is a serious global health problem. Cell culture-based persistent HCV RNA replication systems and infectious HCV production systems are widely used in HCV research. However, persistent HCV production systems have been developed only for HuH-7 hepatoma cells. Here we found a new human hepatoma cell line, Li23, that enables persistent HCV production and anti-HCV reagent assay. Li23's cDNA expression profile differed from HuH-7's, although the two cells had similar liver-specific expression profiles. We used HCV RNA with a specific combination of adaptive mutations to develop an HCV replicon system and genome-length HCV RNA replicating systems including a reporter assay system. Finally, Li23-derived cells persistently produced infectious virus of an HCV strain. Li23-derived cells are potentially useful for understanding the HCV life cycle and for finding antiviral targets.
| Original language | English |
|---|---|
| Pages (from-to) | 41-50 |
| Number of pages | 10 |
| Journal | Virus Research |
| Volume | 146 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Jan 2009 |
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Keywords
- HCV RNA replication
- Hepatitis C virus (HCV)
- Infectious HCV production
- Li23 cells
- Reporter assay for anti-HCV reagents
ASJC Scopus subject areas
- Virology
- Infectious Diseases
- Cancer Research
Cite this
Efficient replication systems for hepatitis C virus using a new human hepatoma cell line. / Kato, Nobuyuki; Mori, Kyoko; Abe, Ken ichi; Dansako, Hiromichi; Kuroki, Misao; Ariumi, Yasuo; Wakita, Takaji; Ikeda, Masanori.
In: Virus Research, Vol. 146, No. 1-2, 01.2009, p. 41-50.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Efficient replication systems for hepatitis C virus using a new human hepatoma cell line
AU - Kato, Nobuyuki
AU - Mori, Kyoko
AU - Abe, Ken ichi
AU - Dansako, Hiromichi
AU - Kuroki, Misao
AU - Ariumi, Yasuo
AU - Wakita, Takaji
AU - Ikeda, Masanori
PY - 2009/1
Y1 - 2009/1
N2 - Persistent hepatitis C virus (HCV) infection causes chronic liver diseases and is a serious global health problem. Cell culture-based persistent HCV RNA replication systems and infectious HCV production systems are widely used in HCV research. However, persistent HCV production systems have been developed only for HuH-7 hepatoma cells. Here we found a new human hepatoma cell line, Li23, that enables persistent HCV production and anti-HCV reagent assay. Li23's cDNA expression profile differed from HuH-7's, although the two cells had similar liver-specific expression profiles. We used HCV RNA with a specific combination of adaptive mutations to develop an HCV replicon system and genome-length HCV RNA replicating systems including a reporter assay system. Finally, Li23-derived cells persistently produced infectious virus of an HCV strain. Li23-derived cells are potentially useful for understanding the HCV life cycle and for finding antiviral targets.
AB - Persistent hepatitis C virus (HCV) infection causes chronic liver diseases and is a serious global health problem. Cell culture-based persistent HCV RNA replication systems and infectious HCV production systems are widely used in HCV research. However, persistent HCV production systems have been developed only for HuH-7 hepatoma cells. Here we found a new human hepatoma cell line, Li23, that enables persistent HCV production and anti-HCV reagent assay. Li23's cDNA expression profile differed from HuH-7's, although the two cells had similar liver-specific expression profiles. We used HCV RNA with a specific combination of adaptive mutations to develop an HCV replicon system and genome-length HCV RNA replicating systems including a reporter assay system. Finally, Li23-derived cells persistently produced infectious virus of an HCV strain. Li23-derived cells are potentially useful for understanding the HCV life cycle and for finding antiviral targets.
KW - HCV RNA replication
KW - Hepatitis C virus (HCV)
KW - Infectious HCV production
KW - Li23 cells
KW - Reporter assay for anti-HCV reagents
UR - http://www.scopus.com/inward/record.url?scp=70350233422&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70350233422&partnerID=8YFLogxK
U2 - 10.1016/j.virusres.2009.08.006
DO - 10.1016/j.virusres.2009.08.006
M3 - Article
C2 - 19720094
AN - SCOPUS:70350233422
VL - 146
SP - 41
EP - 50
JO - Virus Research
JF - Virus Research
SN - 0168-1702
IS - 1-2
ER -