Efficient replication systems for hepatitis C virus using a new human hepatoma cell line

Nobuyuki Kato, Kyoko Mori, Ken ichi Abe, Hiromichi Dansako, Misao Kuroki, Yasuo Ariumi, Takaji Wakita, Masanori Ikeda

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Persistent hepatitis C virus (HCV) infection causes chronic liver diseases and is a serious global health problem. Cell culture-based persistent HCV RNA replication systems and infectious HCV production systems are widely used in HCV research. However, persistent HCV production systems have been developed only for HuH-7 hepatoma cells. Here we found a new human hepatoma cell line, Li23, that enables persistent HCV production and anti-HCV reagent assay. Li23's cDNA expression profile differed from HuH-7's, although the two cells had similar liver-specific expression profiles. We used HCV RNA with a specific combination of adaptive mutations to develop an HCV replicon system and genome-length HCV RNA replicating systems including a reporter assay system. Finally, Li23-derived cells persistently produced infectious virus of an HCV strain. Li23-derived cells are potentially useful for understanding the HCV life cycle and for finding antiviral targets.

Original languageEnglish
Pages (from-to)41-50
Number of pages10
JournalVirus Research
Volume146
Issue number1-2
DOIs
Publication statusPublished - Jan 2009

Fingerprint

Hepacivirus
Hepatocellular Carcinoma
Cell Line
RNA
Hepatovirus
Replicon
Virus Diseases
Virus Replication
Life Cycle Stages
Antiviral Agents
Liver Diseases
Chronic Disease
Complementary DNA
Cell Culture Techniques
Genome
Viruses
Mutation

Keywords

  • HCV RNA replication
  • Hepatitis C virus (HCV)
  • Infectious HCV production
  • Li23 cells
  • Reporter assay for anti-HCV reagents

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Cancer Research

Cite this

Efficient replication systems for hepatitis C virus using a new human hepatoma cell line. / Kato, Nobuyuki; Mori, Kyoko; Abe, Ken ichi; Dansako, Hiromichi; Kuroki, Misao; Ariumi, Yasuo; Wakita, Takaji; Ikeda, Masanori.

In: Virus Research, Vol. 146, No. 1-2, 01.2009, p. 41-50.

Research output: Contribution to journalArticle

Kato, Nobuyuki ; Mori, Kyoko ; Abe, Ken ichi ; Dansako, Hiromichi ; Kuroki, Misao ; Ariumi, Yasuo ; Wakita, Takaji ; Ikeda, Masanori. / Efficient replication systems for hepatitis C virus using a new human hepatoma cell line. In: Virus Research. 2009 ; Vol. 146, No. 1-2. pp. 41-50.
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