Efficient generation of antigen-specific cytotoxic T cells using retrovirally transduced CD40-activated B cells

Eisei Kondo, Max S. Topp, Hans Peter Kiem, Yuichi Obata, Yasuo Morishima, Kiyotaka Kuzushima, Mitsune Tanimoto, Mine Harada, Toshitada Takahashi, Yoshiki Akatsuka

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94 Citations (Scopus)

Abstract

The development of rapid, efficient, and safe methods for generating Ag-specific T cells is necessary for the clinical application of adoptive immunotherapy. We show that B cells stimulated with CD40 ligand and IL-4 (CD40-B cells) can be efficiently transduced with retroviral vectors encoding a model Ag, CMV tegument protein pp65 gene, and maintain high levels of costimulatory molecules after gene transfer. CTL lines specific for pp65 were readily generated in all four healthy CMV-seropositive donors by stimulating autologous CD8+ T cells with these transduced CD40-B cells, both of which were derived from 10 mi peripheral blood. ELISPOT assays revealed that the CTL lines used multiple HLA alleles as restricting elements. Thus, CD40-B cells transduced retrovirally with Ag-encoding cDNA can be potent APC and facilitate to generate Ag-specific CTL in vitro.

Original languageEnglish
Pages (from-to)2164-2171
Number of pages8
JournalJournal of Immunology
Volume169
Issue number4
DOIs
Publication statusPublished - Aug 15 2002

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Kondo, E., Topp, M. S., Kiem, H. P., Obata, Y., Morishima, Y., Kuzushima, K., Tanimoto, M., Harada, M., Takahashi, T., & Akatsuka, Y. (2002). Efficient generation of antigen-specific cytotoxic T cells using retrovirally transduced CD40-activated B cells. Journal of Immunology, 169(4), 2164-2171. https://doi.org/10.4049/jimmunol.169.4.2164