Abstract
We aimed to produce ethanol efficiently by enzymatic saccharification coupled with ethanol fermentation using wheat bran as a biomass. For this purpose, we examined pretreatment conditions of biomass and a combination of commercially available enzyme products for saccharification. When a 3.3% (w/v) wheat bran suspension was pretreated using subcritical water at 140°C with a holding time of 0 h, followed by saccharification at 45°C using a mixture of commercially available Meiselase (composed of cellulase, ce!lobiase/β?- glucosidase, and xylanase) and Novozyme*188 (composed of cellobiase/βS-glucosidase,« amylase, and glucoamylase) at a ratio of 4 to 1 by weight, glucose was released at 10.6 g/l, which corresponded to 90% of the value calculated on the basis of the total amount of glucose-based saccharides contained in the wheat bran. On the other hand, from a pretreated 33% (w/v) wheat bran suspension, 85 g/l glucose was produced, which corresponded to only 72% of the theoretical value, owing to the inhibitory effect of glucose on β?-glucosidase activity. However, when fermentation was carried out at 30°C following 24-h saccharification of the pretreated wheat bran using a mixture of Meiselase and Novozyme*188, the inhibitory effect of glucose was considered to be reduced and as a result, after 21-h fermentation, ethanol was produced at 5.2% (w/v), which corresponded to 88% of the theoretical maximum value.
| Original language | English |
|---|---|
| Pages (from-to) | 216-223 |
| Number of pages | 8 |
| Journal | Seibutsu-kogaku Kaishi |
| Volume | 87 |
| Issue number | 5 |
| Publication status | Published - 2009 |
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Keywords
- Bioethanol
- Enzymatic saccharification
- Ethanol fermentation
- Wheat bran subcritical water treatment
ASJC Scopus subject areas
- Food Science
- Applied Microbiology and Biotechnology
- Biotechnology
Cite this
Efficient ethanol production from wheat bran by enzymatic saccharification using commercially available enzyme products and fermentation using bakers' yeast. / Koreishi, Mayuko; Imanaka, Hiroyuki; Imamura, Koreyoshi; Kariyama, Masahiro; Nakanishi, Kazuhiro.
In: Seibutsu-kogaku Kaishi, Vol. 87, No. 5, 2009, p. 216-223.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Efficient ethanol production from wheat bran by enzymatic saccharification using commercially available enzyme products and fermentation using bakers' yeast
AU - Koreishi, Mayuko
AU - Imanaka, Hiroyuki
AU - Imamura, Koreyoshi
AU - Kariyama, Masahiro
AU - Nakanishi, Kazuhiro
PY - 2009
Y1 - 2009
N2 - We aimed to produce ethanol efficiently by enzymatic saccharification coupled with ethanol fermentation using wheat bran as a biomass. For this purpose, we examined pretreatment conditions of biomass and a combination of commercially available enzyme products for saccharification. When a 3.3% (w/v) wheat bran suspension was pretreated using subcritical water at 140°C with a holding time of 0 h, followed by saccharification at 45°C using a mixture of commercially available Meiselase (composed of cellulase, ce!lobiase/β?- glucosidase, and xylanase) and Novozyme*188 (composed of cellobiase/βS-glucosidase,« amylase, and glucoamylase) at a ratio of 4 to 1 by weight, glucose was released at 10.6 g/l, which corresponded to 90% of the value calculated on the basis of the total amount of glucose-based saccharides contained in the wheat bran. On the other hand, from a pretreated 33% (w/v) wheat bran suspension, 85 g/l glucose was produced, which corresponded to only 72% of the theoretical value, owing to the inhibitory effect of glucose on β?-glucosidase activity. However, when fermentation was carried out at 30°C following 24-h saccharification of the pretreated wheat bran using a mixture of Meiselase and Novozyme*188, the inhibitory effect of glucose was considered to be reduced and as a result, after 21-h fermentation, ethanol was produced at 5.2% (w/v), which corresponded to 88% of the theoretical maximum value.
AB - We aimed to produce ethanol efficiently by enzymatic saccharification coupled with ethanol fermentation using wheat bran as a biomass. For this purpose, we examined pretreatment conditions of biomass and a combination of commercially available enzyme products for saccharification. When a 3.3% (w/v) wheat bran suspension was pretreated using subcritical water at 140°C with a holding time of 0 h, followed by saccharification at 45°C using a mixture of commercially available Meiselase (composed of cellulase, ce!lobiase/β?- glucosidase, and xylanase) and Novozyme*188 (composed of cellobiase/βS-glucosidase,« amylase, and glucoamylase) at a ratio of 4 to 1 by weight, glucose was released at 10.6 g/l, which corresponded to 90% of the value calculated on the basis of the total amount of glucose-based saccharides contained in the wheat bran. On the other hand, from a pretreated 33% (w/v) wheat bran suspension, 85 g/l glucose was produced, which corresponded to only 72% of the theoretical value, owing to the inhibitory effect of glucose on β?-glucosidase activity. However, when fermentation was carried out at 30°C following 24-h saccharification of the pretreated wheat bran using a mixture of Meiselase and Novozyme*188, the inhibitory effect of glucose was considered to be reduced and as a result, after 21-h fermentation, ethanol was produced at 5.2% (w/v), which corresponded to 88% of the theoretical maximum value.
KW - Bioethanol
KW - Enzymatic saccharification
KW - Ethanol fermentation
KW - Wheat bran subcritical water treatment
UR - http://www.scopus.com/inward/record.url?scp=67650835418&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67650835418&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:67650835418
VL - 87
SP - 216
EP - 223
JO - Seibutsu-kogaku Kaishi
JF - Seibutsu-kogaku Kaishi
SN - 0919-3758
IS - 5
ER -