Efficient enhancement of priming effect by intermittent treatment with interferon

Fusao Ikeda; Hiroyuki Shimomura; Hiroshi Nakagawa; Yoshiaki Iwasaki; Masanobu Miyake; Hideyuki Tsuji; Shin ichi Fukioka; Mamoru Itoh; Akira Takahashi; Takao Tsuji

doi:10.1006/bbrc.2000.3843

Efficient enhancement of priming effect by intermittent treatment with interferon

Fusao Ikeda, Hiroyuki Shimomura, Hiroshi Nakagawa, Yoshiaki Iwasaki, Masanobu Miyake, Hideyuki Tsuji, Shin ichi Fukioka, Mamoru Itoh, Akira Takahashi, Takao Tsuji

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2 Citations (Scopus)

Abstract

To improve the efficacy of interferon (IFN) therapy for chronic hepatitis C, we proposed a therapy with twice-a-day injection of IFNβ as the induction. To assess its biological enhancement, we compared antiviral activities in vitro using intermittent treatment schedules simulating the clinical condition. FL cells were treated with IFNβ twice in 12 h interval (Single treatment, 1000 and 0 IU/ml; Double treatment, 500 IU/ml each) and challenged with Sindbis virus. Antiviral activities were determined with 50% cytopathic effect. Activities and mRNA expressions of 2'5'oligoadenylate synthetase (2'5'AS) were also examined. Single treatment showed its peak activity at 9 h, while Double treatment was at 3 h after the second treatment. Double treatment had a significantly higher peak activity. The up-regulated activities of 2'5'AS lasted much longer with Double treatment. The present findings demonstrated Double treatment could induce efficient biological enhancement, which is thought based on the priming effect. (C) 2000 Academic Press.

Original language	English
Pages (from-to)	599-603
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	278
Issue number	3
DOIs	https://doi.org/10.1006/bbrc.2000.3843
Publication status	Published - Nov 30 2000

Keywords

Antiviral effect
Chronic hepatitis C
Interferon
Intermittent treatment
Priming effect

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1006/bbrc.2000.3843

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abstract = "To improve the efficacy of interferon (IFN) therapy for chronic hepatitis C, we proposed a therapy with twice-a-day injection of IFNβ as the induction. To assess its biological enhancement, we compared antiviral activities in vitro using intermittent treatment schedules simulating the clinical condition. FL cells were treated with IFNβ twice in 12 h interval (Single treatment, 1000 and 0 IU/ml; Double treatment, 500 IU/ml each) and challenged with Sindbis virus. Antiviral activities were determined with 50% cytopathic effect. Activities and mRNA expressions of 2'5'oligoadenylate synthetase (2'5'AS) were also examined. Single treatment showed its peak activity at 9 h, while Double treatment was at 3 h after the second treatment. Double treatment had a significantly higher peak activity. The up-regulated activities of 2'5'AS lasted much longer with Double treatment. The present findings demonstrated Double treatment could induce efficient biological enhancement, which is thought based on the priming effect. (C) 2000 Academic Press.",

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author = "Fusao Ikeda and Hiroyuki Shimomura and Hiroshi Nakagawa and Yoshiaki Iwasaki and Masanobu Miyake and Hideyuki Tsuji and Fukioka, {Shin ichi} and Mamoru Itoh and Akira Takahashi and Takao Tsuji",

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AU - Ikeda, Fusao

AU - Shimomura, Hiroyuki

AU - Nakagawa, Hiroshi

AU - Iwasaki, Yoshiaki

AU - Miyake, Masanobu

AU - Tsuji, Hideyuki

AU - Fukioka, Shin ichi

AU - Itoh, Mamoru

AU - Takahashi, Akira

AU - Tsuji, Takao

PY - 2000/11/30

Y1 - 2000/11/30

N2 - To improve the efficacy of interferon (IFN) therapy for chronic hepatitis C, we proposed a therapy with twice-a-day injection of IFNβ as the induction. To assess its biological enhancement, we compared antiviral activities in vitro using intermittent treatment schedules simulating the clinical condition. FL cells were treated with IFNβ twice in 12 h interval (Single treatment, 1000 and 0 IU/ml; Double treatment, 500 IU/ml each) and challenged with Sindbis virus. Antiviral activities were determined with 50% cytopathic effect. Activities and mRNA expressions of 2'5'oligoadenylate synthetase (2'5'AS) were also examined. Single treatment showed its peak activity at 9 h, while Double treatment was at 3 h after the second treatment. Double treatment had a significantly higher peak activity. The up-regulated activities of 2'5'AS lasted much longer with Double treatment. The present findings demonstrated Double treatment could induce efficient biological enhancement, which is thought based on the priming effect. (C) 2000 Academic Press.

AB - To improve the efficacy of interferon (IFN) therapy for chronic hepatitis C, we proposed a therapy with twice-a-day injection of IFNβ as the induction. To assess its biological enhancement, we compared antiviral activities in vitro using intermittent treatment schedules simulating the clinical condition. FL cells were treated with IFNβ twice in 12 h interval (Single treatment, 1000 and 0 IU/ml; Double treatment, 500 IU/ml each) and challenged with Sindbis virus. Antiviral activities were determined with 50% cytopathic effect. Activities and mRNA expressions of 2'5'oligoadenylate synthetase (2'5'AS) were also examined. Single treatment showed its peak activity at 9 h, while Double treatment was at 3 h after the second treatment. Double treatment had a significantly higher peak activity. The up-regulated activities of 2'5'AS lasted much longer with Double treatment. The present findings demonstrated Double treatment could induce efficient biological enhancement, which is thought based on the priming effect. (C) 2000 Academic Press.

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KW - Chronic hepatitis C

KW - Interferon

KW - Intermittent treatment

KW - Priming effect

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Efficient enhancement of priming effect by intermittent treatment with interferon

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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