Efficient embryo transfer in the common marmoset monkey (Callithrix jacchus) with a reduced transfer volume: A non-surgical approach with cryopreserved late-stage embryos1

Hidetoshi Ishibashi, Hideyuki H. Motohashi, Mami Kumon, Kazuhiro Yamamoto, Hironori Okada, Takashi Okada, Kazuhiko Seki

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Among primates, the common marmoset is suitable for primate embryology research. Its small body size, however, has delayed the technical development of efficient embryo transfer. Furthermore, three factors have been determined to adversely affect the performance of marmoset embryo transfer: nonsurgical approaches, the use of cryopreserved embryos, and the use of late-stage embryos. Here we performed embryo transfer under conditions that included the above three factors and using either a small (1 ll or less) or a large volume (2-3 ll) of medium. The pregnancy and birth rates were 50% (5/10) and 27% (3/11), respectively, when using the large volume, and 80% (8/10) and 75% (9/12), respectively, when using the small volume. The latter scores exceed those of previous reports using comparable conditions. Thus, it appears that these three previously considered factors could be overcome, and we propose that reducing the transfer volume to 1 ll or less is essential for successful marmoset embryo transfer.

Original languageEnglish
Article numberArticle 115
JournalBiology of reproduction
Volume88
Issue number5
DOIs
Publication statusPublished - 2013

Keywords

  • Birth weight
  • Cryopreservation
  • Double embryo transfer
  • Endometrium
  • Litter size

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology

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