Efficient elimination of multidrug-resistant Staphylococcus aureus by cloned lysin derived from bacteriophage φMR11

Mohammad Rashel, Jumpei Uchiyama, Takako Ujihara, Yoshio Uehara, Shu Kuramoto, Shigeyoshi Sugihara, Ken Ichi Yagyu, Asako Muraoka, Motoyuki Sugai, Keiichi Hiramatsu, Koichi Honke, Shigenobu Matsuzaki

Research output: Contribution to journalArticlepeer-review

171 Citations (Scopus)

Abstract

We report the successful purification of a cloned lysin encoded by the novel Staphylococcus aureus bacteriophage φMR11. The lysin, designated MV-L, rapidly and completely lysed cells of a number of S. aureus strains tested, including methicillin-resistant S. aureus (MRSA) and vancomycin-resistant S. aureus and a subset of vancomycin-intermediate S. aureus (VISA) in growing conditions. MV-L-mediated killing is specific to S. aureus and not to other species, except for S. simulans. MV-L exerted its staphylocidal effect synergistically with glycopeptide antibiotics against VISA. MV-L efficiently eliminated MRSA that had been artificially inoculated into the nares of mice. The intraperitoneal administration of MV-L also protected mice against MRSA septic death, without any harmful effects. Although MV-L evoked detectable levels of a humoral response in mice, the antibodies did not abolish the bacteriolytic activity. These results indicate that MV-L might be useful as a powerful therapeutic agent against multidrug-resistant S. aureus infections.

Original languageEnglish
Pages (from-to)1237-1247
Number of pages11
JournalJournal of Infectious Diseases
Volume196
Issue number8
DOIs
Publication statusPublished - 2007
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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