TY - JOUR
T1 - Efficient Cre/loxP site-specific recombination in a HepG2 human liver cell line
AU - Kobayashi, Naoya
AU - Noguchi, Hirofumi
AU - Westerman, Karen A.
AU - Matsumura, Toshihisa
AU - Watanabe, Takamasa
AU - Totsugawa, Toshinori
AU - Fujiwara, Toshiyoshi
AU - Leboulch, Philippe
AU - Tanaka, Noriaki
PY - 2000/1/1
Y1 - 2000/1/1
N2 - A worldwide shortage of donor livers is a limiting factor of the clinical application of hepatocyte transplantation (HTX). To resolve this issue, we focused on a reversible immortalization system that allows temporary expansion of primary hepatocyte populations by transfer of an oncogene that can be subsequently excised. As a preliminary test toward this goal, we examined the efficacy of Cre/loxP site-specific recombination in a transformed human liver cell line, HepG2. The present study utilized retroviral transfer of a prototypical immortalizing gene, simian virus 40 large T antigen (SV40Tag), flanked by a pair of loxP recombination targets and adenovirus-mediated Cre/loxP recombination. Here we report that complete elimination of the retroviral transferred oncogene was achieved by site-specific recombination using a replication-deficient recombinant adenovirus vector producing Cre recombinase (Ad-Cre).
AB - A worldwide shortage of donor livers is a limiting factor of the clinical application of hepatocyte transplantation (HTX). To resolve this issue, we focused on a reversible immortalization system that allows temporary expansion of primary hepatocyte populations by transfer of an oncogene that can be subsequently excised. As a preliminary test toward this goal, we examined the efficacy of Cre/loxP site-specific recombination in a transformed human liver cell line, HepG2. The present study utilized retroviral transfer of a prototypical immortalizing gene, simian virus 40 large T antigen (SV40Tag), flanked by a pair of loxP recombination targets and adenovirus-mediated Cre/loxP recombination. Here we report that complete elimination of the retroviral transferred oncogene was achieved by site-specific recombination using a replication-deficient recombinant adenovirus vector producing Cre recombinase (Ad-Cre).
KW - Cre/loxP site-specific recombination
KW - HepG2 cells
KW - Reversible immortalization
UR - http://www.scopus.com/inward/record.url?scp=0034529735&partnerID=8YFLogxK
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U2 - 10.1177/096368970000900525
DO - 10.1177/096368970000900525
M3 - Article
C2 - 11144976
AN - SCOPUS:0034529735
VL - 9
SP - 737
EP - 742
JO - Cell Transplantation
JF - Cell Transplantation
SN - 0963-6897
IS - 5
ER -