Efficient callus induction and shoot regeneration by anther culture in male sterile mutants of tomato (Lycopersicon esculentum Mill. cv. First)

Youhui Ma, Kenji Kato, Masaharu Masuda

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Anthers of three male sterile mutants of tomato plants and their original cv. First were subjected to several factors which affected callus induction and shoot regeneration. Callus induction capacity was significantly improved by the presence of ms genes which disrupt microsporogenesis at meiosis in the anthers of Fms-2 line. Fms-1 and Fms-3, which block microsporogenesis at microspore and tetrad formation respectively, were inferior to Fms-2 in callus induction. Experiments carried out with the Fms-2 line demonstrated that the developmental stage of the explant was a crucial factor in callus formation and that optimum stage was between pre- meiosis and metaphase I, which corresponds to the stage just after the blockage of microsporogenesis. Callus was efficiently induced from anthers, pretreated at 4 °C for 3 days, on MS medium, supplemented with 5.0 mg · liter-1 IAA and 2.5 mg · liter-1 zeatin, under a 16-hr photoperiod. The highest frequency of shoot differentiation was achieved on MS medium, supplemented with 0.2 mg · liter-1 kinetin.

Original languageEnglish
Pages (from-to)768-773
Number of pages6
JournalJournal of the Japanese Society for Horticultural Science
Volume68
Issue number4
Publication statusPublished - Jul 1 1999

Keywords

  • Anther culture
  • Callus induction
  • Male sterile tomato
  • Shoot differentiation

ASJC Scopus subject areas

  • Horticulture

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