Efficient bone formation in a swine socket lift model using escherichia coli-derived recombinant human bone morphogenetic protein-2 adsorbed in β-tricalcium phosphate

Mitsuaki Ono, Wataru Sonoyama, Katushi Yamamoto, Yasutaka Oida, Kentaro Akiyama, Shigehiko Shinkawa, Ryu Nakajima, Hai T. Pham, Emilio satoshi Hara, Takuo Kuboki

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Several preclinical studies have shown that Escherichia coliderived bone morphogenetic protein-2 (E-BMP-2) is as effective as mammalian cell-derived bone morphogenetic protein- 2 (C-BMP-2) in the treatment of bone defects. However, further investigation of the effectiveness and determination of the optimal dosage of E-BMP-2 in large animals are still necessary before its full application in humans. This study investigated the efficiency of different concentrations of EBMP- 2 adsorbed in β-TCP for bone augmentation and osseointegration of immediate dental implants in a swine socket lift model. Following exposure of the maxillary sinus lateral wall, a 3.4-mm (diameter) cavity was drilled and filled with 0.1 g of β-TCP containing different doses of E-BMP-2 (0, 10, 30, or 100 ?g/site) to lift the Schneiderian membrane. A dental implant was then immediately inserted. Bone-to-implant contact (BIC) and bone density (BD) examined via histological analysis were used as parameters to assess E-BMP-2 efficiency in bone formation. The implant stability quotient (ISQ) was measured using Osstell to determine the effect of E-BMP-2/β-TCP on implant stability. After 8 weeks, the groups that received 30 and 100 ?g of E-BMP-2 showed substantial new bone formation in the elevated space, while no bone formation was observed with β-TCP alone. Accordingly, BIC and BD presented a dose-dependent response to increasing doses of E-BMP-2. However, there was no increase in implant stability with E-BMP-2 treatment. In conclusion, the E-BMP-2/β-TCP combination was efficient in bone formation and osseointegration of dental implants in a socket lift model in mini-pigs.

Original languageEnglish
Pages (from-to)249-255
Number of pages7
JournalCells Tissues Organs
Volume199
Issue number4
DOIs
Publication statusPublished - Feb 24 2014

Fingerprint

Bone Morphogenetic Protein 2
Escherichia
Osteogenesis
Swine
Escherichia coli
Dental Implants
Osseointegration
Bone and Bones
Bone Density
recombinant human bone morphogenetic protein-2
tricalcium phosphate
Maxillary Sinus
Nasal Mucosa

Keywords

  • Biomaterials
  • Bone density
  • Bone regeneration
  • Bone-to-implant contact
  • Escherichia coli-derived recombinant human bone morphogenetic protein-2
  • Implant
  • Sinus lift
  • Surgery
  • β-Tricalcium phosphate

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Medicine(all)

Cite this

Efficient bone formation in a swine socket lift model using escherichia coli-derived recombinant human bone morphogenetic protein-2 adsorbed in β-tricalcium phosphate. / Ono, Mitsuaki; Sonoyama, Wataru; Yamamoto, Katushi; Oida, Yasutaka; Akiyama, Kentaro; Shinkawa, Shigehiko; Nakajima, Ryu; Pham, Hai T.; Hara, Emilio satoshi; Kuboki, Takuo.

In: Cells Tissues Organs, Vol. 199, No. 4, 24.02.2014, p. 249-255.

Research output: Contribution to journalArticle

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abstract = "Several preclinical studies have shown that Escherichia coliderived bone morphogenetic protein-2 (E-BMP-2) is as effective as mammalian cell-derived bone morphogenetic protein- 2 (C-BMP-2) in the treatment of bone defects. However, further investigation of the effectiveness and determination of the optimal dosage of E-BMP-2 in large animals are still necessary before its full application in humans. This study investigated the efficiency of different concentrations of EBMP- 2 adsorbed in β-TCP for bone augmentation and osseointegration of immediate dental implants in a swine socket lift model. Following exposure of the maxillary sinus lateral wall, a 3.4-mm (diameter) cavity was drilled and filled with 0.1 g of β-TCP containing different doses of E-BMP-2 (0, 10, 30, or 100 ?g/site) to lift the Schneiderian membrane. A dental implant was then immediately inserted. Bone-to-implant contact (BIC) and bone density (BD) examined via histological analysis were used as parameters to assess E-BMP-2 efficiency in bone formation. The implant stability quotient (ISQ) was measured using Osstell to determine the effect of E-BMP-2/β-TCP on implant stability. After 8 weeks, the groups that received 30 and 100 ?g of E-BMP-2 showed substantial new bone formation in the elevated space, while no bone formation was observed with β-TCP alone. Accordingly, BIC and BD presented a dose-dependent response to increasing doses of E-BMP-2. However, there was no increase in implant stability with E-BMP-2 treatment. In conclusion, the E-BMP-2/β-TCP combination was efficient in bone formation and osseointegration of dental implants in a socket lift model in mini-pigs.",
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AU - Oida, Yasutaka

AU - Akiyama, Kentaro

AU - Shinkawa, Shigehiko

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AB - Several preclinical studies have shown that Escherichia coliderived bone morphogenetic protein-2 (E-BMP-2) is as effective as mammalian cell-derived bone morphogenetic protein- 2 (C-BMP-2) in the treatment of bone defects. However, further investigation of the effectiveness and determination of the optimal dosage of E-BMP-2 in large animals are still necessary before its full application in humans. This study investigated the efficiency of different concentrations of EBMP- 2 adsorbed in β-TCP for bone augmentation and osseointegration of immediate dental implants in a swine socket lift model. Following exposure of the maxillary sinus lateral wall, a 3.4-mm (diameter) cavity was drilled and filled with 0.1 g of β-TCP containing different doses of E-BMP-2 (0, 10, 30, or 100 ?g/site) to lift the Schneiderian membrane. A dental implant was then immediately inserted. Bone-to-implant contact (BIC) and bone density (BD) examined via histological analysis were used as parameters to assess E-BMP-2 efficiency in bone formation. The implant stability quotient (ISQ) was measured using Osstell to determine the effect of E-BMP-2/β-TCP on implant stability. After 8 weeks, the groups that received 30 and 100 ?g of E-BMP-2 showed substantial new bone formation in the elevated space, while no bone formation was observed with β-TCP alone. Accordingly, BIC and BD presented a dose-dependent response to increasing doses of E-BMP-2. However, there was no increase in implant stability with E-BMP-2 treatment. In conclusion, the E-BMP-2/β-TCP combination was efficient in bone formation and osseointegration of dental implants in a socket lift model in mini-pigs.

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