Efficient assay for total antioxidant capacity in human plasma using a 96-well microplte

Yasuhiro Kambayashi, Nguyen Thanh Binh, Hiroki W. Asakura, Yuri Hibino, Yoshiaki Hitomi, Hiroyuki Nakamura, Keiki Ogino

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64 Citations (Scopus)

Abstract

In the present study, we tried to establish an efficient assay for total antioxidant capacity (TAC) in human plasma using a 96-well microplate. TAC was assessed using lag time by antioxidants against the myoglobin-induced oxidation of 2,2′-azino-di(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) with hydrogen peroxide, and expressed as Trolox equivalent. The linearity of the calibration curve with Trolox was maintained with the Trolox concentration range from 2.5 μM to 25 μM (R2 = 0.997). The assay was applied to the measurement of TAC in healthy human plasma. Coefficient of variation in intraday assay was 2.4%. Difference was not observed in interday assay. Plasma TAC of men ((569 ± 41) μM Trolox equivalent; n = 6) was higher than that of women ((430 ± 28) μM Trolox equivalent; n = 4). After the vegetable juice was drunk for 1 week, the increase in plasma TAC was observed in almost all the volunteers. In summary, we developed the efficient assay for plasma TAC using a 96-well microplate.

Original languageEnglish
Pages (from-to)46-51
Number of pages6
Journaljournal of clinical biochemistry and nutrition
Volume44
Issue number1
DOIs
Publication statusPublished - Jan 2009

Keywords

  • ABTS
  • Human plasma
  • Lag time
  • Microplate
  • Total antioxidant capacity

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics
  • Clinical Biochemistry

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    Kambayashi, Y., Binh, N. T., Asakura, H. W., Hibino, Y., Hitomi, Y., Nakamura, H., & Ogino, K. (2009). Efficient assay for total antioxidant capacity in human plasma using a 96-well microplte. journal of clinical biochemistry and nutrition, 44(1), 46-51. https://doi.org/10.3164/jcbn.08-162