Efficacy of Tumor-Targeting Salmonella Typhimurium A1-R on Nude Mouse Models of Metastatic and Disseminated Human Ovarian Cancer

Yasunori Matsumoto, Shinji Miwa, Yong Zhang, Yukihiko Hiroshima, Shuuya Yano, Fuminari Uehara, Mako Yamamoto, Makoto Toneri, Michael Bouvet, Hisahiro Matsubara, Robert M. Hoffman, Ming Zhao

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Abstract

We report here the efficacy of tumor-targeting Salmonella typhimurium A1-R (A1-R) on mouse models of disseminated and metastatic ovarian cancer. The proliferation-inhibitory efficacy of A1-R on human ovarian cancer cell lines (SKOV-3-GFP, OVCAR-3-RFP) was initially demonstrated in vitro. Orthotopic and dissemination mouse models of ovarian cancer were made with the human ovarian cancer cell line SKOV-3-GFP. After tumor implantation, the mice were treated with A1-R (5 × 107 colony-forming units [CFU], i.v.), and there were no severe adverse events observed. In the orthotopic model, tumor volume after treatment was 276 ± 60.8 mm3, compared to 930 ± 342 mm3 in the untreated control group (P = 0.022). There was also a significant difference in survival between treated mice and untreated mice in a peritoneal dissemination model (P = 0.005). The results of this report demonstrate that A1-R is effective for highly aggressive human ovarian cancer in metastatic and dissemination mouse models and suggest its clinical potential for this highly treatment-resistant disease.

Original languageEnglish
Pages (from-to)1996-2003
Number of pages8
JournalJournal of Cellular Biochemistry
Volume115
Issue number11
DOIs
Publication statusPublished - Nov 1 2014
Externally publishedYes

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Keywords

  • BACTERIA
  • GFP
  • IMAGING
  • NUDE MICE
  • OVARIAN CANCER
  • Salmonella typhimurium
  • TUMOR-TARGETING

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Matsumoto, Y., Miwa, S., Zhang, Y., Hiroshima, Y., Yano, S., Uehara, F., Yamamoto, M., Toneri, M., Bouvet, M., Matsubara, H., Hoffman, R. M., & Zhao, M. (2014). Efficacy of Tumor-Targeting Salmonella Typhimurium A1-R on Nude Mouse Models of Metastatic and Disseminated Human Ovarian Cancer. Journal of Cellular Biochemistry, 115(11), 1996-2003. https://doi.org/10.1002/jcb.24871