Efficacy of pretransection of peripheral nerve for promoting the survival of cografted chromaffin cells and recovery of host dopaminergic fibers in animal models of Parkinson's disease

Isao Date, Yasuyuki Miyoshi, Takashi Imaoka, Tomohisa Furuta, Shoji Asari, Takashi Ohmoto

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Nerve growth factor (NGF) concentration in the distal stump of the transected peripheral nerve has been shown to increase more than 20 times one day after transection. We performed adrenal medullary alone grafts or cografts of adrenal medulla and acutely transected or pretransected (24 h before) sciatic nerve into the striatum of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice, and compared the survival of chromaffin cells and the recovery of the host-intrinsic dopaminergic fibers using tyrosine hydroxylase immunocytochemistry and high-performance liquid chromatography. We also performed peripheral nerve alone grafting (acutely transected or pretransected) for comparison. Adrenal medullary chromaffin cells cografted with pretransected sciatic nerve survived better than those in adrenal grafts alone or those cografted with acutely transected sciatic nerve. Host dopaminergic fiber recovery was also most prominent in mice cografted with pretransected peripheral nerve. Animals receiving grafts of peripheral nerve alone showed limited recovery of host dopaminergic fibers and the degree of recovery was lower than that of animals receiving cografts of adrenal medulla with pretransected peripheral nerve. We conclude that pretransected peripheral nerve enhanced the survival of cografted chromaffin cells and this increased survival led to promote the recovery of host-intrinsic dopaminergic fibers. This grafting procedure might be promising in application to patients with Parkinson's disease.

Original languageEnglish
Pages (from-to)213-221
Number of pages9
JournalNeuroscience Research
Volume20
Issue number3
DOIs
Publication statusPublished - 1994

Fingerprint

Chromaffin Cells
Peripheral Nerves
Parkinson Disease
Animal Models
Sciatic Nerve
Adrenal Medulla
Transplants
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
Tyrosine 3-Monooxygenase
Nerve Growth Factor
Cell Survival
Immunohistochemistry
High Pressure Liquid Chromatography

Keywords

  • Adrenal medulla
  • Cograft
  • MPTP
  • Neural transplantation
  • Parkinson's disease
  • Peripheral nerve

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Efficacy of pretransection of peripheral nerve for promoting the survival of cografted chromaffin cells and recovery of host dopaminergic fibers in animal models of Parkinson's disease. / Date, Isao; Miyoshi, Yasuyuki; Imaoka, Takashi; Furuta, Tomohisa; Asari, Shoji; Ohmoto, Takashi.

In: Neuroscience Research, Vol. 20, No. 3, 1994, p. 213-221.

Research output: Contribution to journalArticle

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