Efficacy of hypochlorous acid scavengers in the prevention of protein carbonyl formation

Liang Jun Yan, Maret G. Traber, Hirotsugu Kobuchi, Seiichi Matsugo, Hans J. Tritschler, Lester Packer

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

We observed that protein (bovine serum albumin) carbonyl content increases upon hypochlorite oxidation, and this increase is inhibited in a concentration-dependent manner in the presence of hypochlorite scavengers. Based on this observation, we tested whether some known hypochlorite scavengers (lipoic acid, cysteine, and glutathione) and some other antioxidants (uric acid, ascorbic acid, α-tocopherol, and probucol) could prevent protein carbonyl formation. N-acetylcysteine, dihydrolipoic acid, cysteine, and glutathione (reduced form, GSH), which otherwise could not be tested in a previously reported 5-thio-2-nitro-benzoic acid test system, were successfully evaluated in our assay. The hypochlorite scavenging capacity of different compounds, compared by determining the IC50 (concentration which produces 50% inhibition), showed that the compounds tested have the following potency: dihydrolipoic acid > GSH, N-acetylcysteine > cysteine > S-methyl glutathione > lipoic acid, ascorbic acid > cystine, GSSG, and uric acid. No scavenging ability was observed for either α-tocopherol or probucol.

Original languageEnglish
Pages (from-to)330-334
Number of pages5
JournalArchives of Biochemistry and Biophysics
Volume327
Issue number2
DOIs
Publication statusPublished - Mar 15 1996
Externally publishedYes

Keywords

  • bovine serum albumin (BSA)
  • hypochlorous acid
  • protein carbonyls
  • scavengers

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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