Effects on genome constitution and novel cell wall formation caused by the addition of 5RS rye chromosome to common wheat

Zhi Jun Cheng, Minoru Murata, [No Value] Sodmergen, Xiao Mei Li, Hai Nian, Jian Min Wan

Research output: Contribution to journalArticle

Abstract

The cytological instability of common wheat-rye addition lines was investigated in the present study. The chromosome numbers of almost all addition lines were considerably stable, but those of CS+5R were very variable. The rye chromosome added in this line was found to be much shorter than expected. Fluorescent in situ hybridization with 5S rDNA and the centromere-specific probes clearly revealed that the short rye chromosome contains only a short arm of chromosome 5R (5RS). In this line, chromosome numbers of both 5RS and common wheat were changeable. The chromosome numbers ranged from 2n=36 to 2n=44 in the cells carrying two 5RS, and ranged from 2n=31 to 2n=44 in one 5RS cells. In addition to the chromosome instability, the multicells wrapped in a sac-like structure were frequently observed in the root meristematic tissues of CS+5RS after the enzyme treatment for chromosome preparation. Genomic in situ hybridization with rye DNA as a probe showed that all cells in sacs investigated were at the interphase stage and contained one or two 5RS chromosomes. An electron microscopic analysis revealed that the cells of CS+5RS, particularly in sacs, have abnormal (irregular and curved) cell walls. These results indicate that 5RS has (a) specific factor(s) influencing the cell wall development as well as the genome stability.

Original languageEnglish
Pages (from-to)503-509
Number of pages7
JournalJournal of Integrative Plant Biology
Volume50
Issue number4
DOIs
Publication statusPublished - Apr 2008

Fingerprint

Constitution and Bylaws
Chromosomes
rye
Cell Wall
Triticum
Genes
Cells
cell walls
Genome
chromosomes
wheat
genome
chromosome number
probes (equipment)
cells
enzymatic treatment
centromeres
fluorescence in situ hybridization
interphase
Chromosomal Instability

Keywords

  • Addition lines
  • Cell wall
  • Chromosome instability
  • Common wheat
  • Rye

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Effects on genome constitution and novel cell wall formation caused by the addition of 5RS rye chromosome to common wheat. / Cheng, Zhi Jun; Murata, Minoru; Sodmergen, [No Value]; Li, Xiao Mei; Nian, Hai; Wan, Jian Min.

In: Journal of Integrative Plant Biology, Vol. 50, No. 4, 04.2008, p. 503-509.

Research output: Contribution to journalArticle

Cheng, Zhi Jun ; Murata, Minoru ; Sodmergen, [No Value] ; Li, Xiao Mei ; Nian, Hai ; Wan, Jian Min. / Effects on genome constitution and novel cell wall formation caused by the addition of 5RS rye chromosome to common wheat. In: Journal of Integrative Plant Biology. 2008 ; Vol. 50, No. 4. pp. 503-509.
@article{d9edbd4cdac74fd88fbc92c02579d33b,
title = "Effects on genome constitution and novel cell wall formation caused by the addition of 5RS rye chromosome to common wheat",
abstract = "The cytological instability of common wheat-rye addition lines was investigated in the present study. The chromosome numbers of almost all addition lines were considerably stable, but those of CS+5R were very variable. The rye chromosome added in this line was found to be much shorter than expected. Fluorescent in situ hybridization with 5S rDNA and the centromere-specific probes clearly revealed that the short rye chromosome contains only a short arm of chromosome 5R (5RS). In this line, chromosome numbers of both 5RS and common wheat were changeable. The chromosome numbers ranged from 2n=36 to 2n=44 in the cells carrying two 5RS, and ranged from 2n=31 to 2n=44 in one 5RS cells. In addition to the chromosome instability, the multicells wrapped in a sac-like structure were frequently observed in the root meristematic tissues of CS+5RS after the enzyme treatment for chromosome preparation. Genomic in situ hybridization with rye DNA as a probe showed that all cells in sacs investigated were at the interphase stage and contained one or two 5RS chromosomes. An electron microscopic analysis revealed that the cells of CS+5RS, particularly in sacs, have abnormal (irregular and curved) cell walls. These results indicate that 5RS has (a) specific factor(s) influencing the cell wall development as well as the genome stability.",
keywords = "Addition lines, Cell wall, Chromosome instability, Common wheat, Rye",
author = "Cheng, {Zhi Jun} and Minoru Murata and Sodmergen, {[No Value]} and Li, {Xiao Mei} and Hai Nian and Wan, {Jian Min}",
year = "2008",
month = "4",
doi = "10.1111/j.1744-7909.2008.00639.x",
language = "English",
volume = "50",
pages = "503--509",
journal = "Journal of Integrative Plant Biology",
issn = "1672-9072",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Effects on genome constitution and novel cell wall formation caused by the addition of 5RS rye chromosome to common wheat

AU - Cheng, Zhi Jun

AU - Murata, Minoru

AU - Sodmergen, [No Value]

AU - Li, Xiao Mei

AU - Nian, Hai

AU - Wan, Jian Min

PY - 2008/4

Y1 - 2008/4

N2 - The cytological instability of common wheat-rye addition lines was investigated in the present study. The chromosome numbers of almost all addition lines were considerably stable, but those of CS+5R were very variable. The rye chromosome added in this line was found to be much shorter than expected. Fluorescent in situ hybridization with 5S rDNA and the centromere-specific probes clearly revealed that the short rye chromosome contains only a short arm of chromosome 5R (5RS). In this line, chromosome numbers of both 5RS and common wheat were changeable. The chromosome numbers ranged from 2n=36 to 2n=44 in the cells carrying two 5RS, and ranged from 2n=31 to 2n=44 in one 5RS cells. In addition to the chromosome instability, the multicells wrapped in a sac-like structure were frequently observed in the root meristematic tissues of CS+5RS after the enzyme treatment for chromosome preparation. Genomic in situ hybridization with rye DNA as a probe showed that all cells in sacs investigated were at the interphase stage and contained one or two 5RS chromosomes. An electron microscopic analysis revealed that the cells of CS+5RS, particularly in sacs, have abnormal (irregular and curved) cell walls. These results indicate that 5RS has (a) specific factor(s) influencing the cell wall development as well as the genome stability.

AB - The cytological instability of common wheat-rye addition lines was investigated in the present study. The chromosome numbers of almost all addition lines were considerably stable, but those of CS+5R were very variable. The rye chromosome added in this line was found to be much shorter than expected. Fluorescent in situ hybridization with 5S rDNA and the centromere-specific probes clearly revealed that the short rye chromosome contains only a short arm of chromosome 5R (5RS). In this line, chromosome numbers of both 5RS and common wheat were changeable. The chromosome numbers ranged from 2n=36 to 2n=44 in the cells carrying two 5RS, and ranged from 2n=31 to 2n=44 in one 5RS cells. In addition to the chromosome instability, the multicells wrapped in a sac-like structure were frequently observed in the root meristematic tissues of CS+5RS after the enzyme treatment for chromosome preparation. Genomic in situ hybridization with rye DNA as a probe showed that all cells in sacs investigated were at the interphase stage and contained one or two 5RS chromosomes. An electron microscopic analysis revealed that the cells of CS+5RS, particularly in sacs, have abnormal (irregular and curved) cell walls. These results indicate that 5RS has (a) specific factor(s) influencing the cell wall development as well as the genome stability.

KW - Addition lines

KW - Cell wall

KW - Chromosome instability

KW - Common wheat

KW - Rye

UR - http://www.scopus.com/inward/record.url?scp=41949132060&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=41949132060&partnerID=8YFLogxK

U2 - 10.1111/j.1744-7909.2008.00639.x

DO - 10.1111/j.1744-7909.2008.00639.x

M3 - Article

C2 - 18713385

AN - SCOPUS:41949132060

VL - 50

SP - 503

EP - 509

JO - Journal of Integrative Plant Biology

JF - Journal of Integrative Plant Biology

SN - 1672-9072

IS - 4

ER -