Abstract
We examined the attachment of Staphylococcus aureus to plastic tissue- culture coverslips after incubation for 24 h. The attachment to coverslips was weaker in rabbit plasma with 5% zinc oxide (ZnO) than in the control rabbit plasma without ZnO (P <0.01). Plasma coagulation by S. aureus strains was not detected in plasma with 5% ZnO after incubation for 24 h. The membranous structure (an immature biofilm) was formed on the coverslips by S. aureus cells in plasma after incubation for 24 h. The colony counts of S. aureus cells on the membranous structures were lower in plasma with 5% ZnO, plasma with 0.2% hinokitiol, plasma with 5% ZnO + 0.2% hinokitiol, plasma with cefdinir at 4 minimum inhibitory concentration (MIC) and plasma with levofloxacin at 4 MIC, than in the control plasma after incubation for 24 h (P <0.01). The colonies on the membranous structures completely disappeared in the case of plasma with 5% ZnO and 0.2% hinokitiol. The colony counts on membranous structures were lower in plasma with cefdinir at 4 MIC or levofloxacin at 4 MIC containing 5% ZnO than in plasma with cefdinir at 4 MIC or levofloxacin at 4 MIC only, (P <0.05). The MICs of hinokitiol against S. aureus strains peaked at an MIC distribution of 16-32 μg/ml. The peak shifted to below 1 μg/ml by adding 5% ZnO in agar plate method. The results suggest that the attachment of S. aureus cells to the coverslips is suppressed in the presence of 5% ZnO and that antistaphylococcal activities of cefdinir, levofloxacin and hinokitiol increase in the presence of 5% ZnO.
| Original language | English |
|---|---|
| Pages (from-to) | 67-74 |
| Number of pages | 8 |
| Journal | Journal of Dermatological Science |
| Volume | 17 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - May 1998 |
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Keywords
- Atopic dermatitis
- Attachment
- Staphylococcus aureus
- Zinc oxide
ASJC Scopus subject areas
- Dermatology
Cite this
Effects of zinc oxide on the attachment of Staphylococcus aureus strains. / Akiyama, Hisanori; Yamasaki, Osamu; Kanzaki, Hiroko; Tada, Joji; Arata, Jirô.
In: Journal of Dermatological Science, Vol. 17, No. 1, 05.1998, p. 67-74.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of zinc oxide on the attachment of Staphylococcus aureus strains
AU - Akiyama, Hisanori
AU - Yamasaki, Osamu
AU - Kanzaki, Hiroko
AU - Tada, Joji
AU - Arata, Jirô
PY - 1998/5
Y1 - 1998/5
N2 - We examined the attachment of Staphylococcus aureus to plastic tissue- culture coverslips after incubation for 24 h. The attachment to coverslips was weaker in rabbit plasma with 5% zinc oxide (ZnO) than in the control rabbit plasma without ZnO (P <0.01). Plasma coagulation by S. aureus strains was not detected in plasma with 5% ZnO after incubation for 24 h. The membranous structure (an immature biofilm) was formed on the coverslips by S. aureus cells in plasma after incubation for 24 h. The colony counts of S. aureus cells on the membranous structures were lower in plasma with 5% ZnO, plasma with 0.2% hinokitiol, plasma with 5% ZnO + 0.2% hinokitiol, plasma with cefdinir at 4 minimum inhibitory concentration (MIC) and plasma with levofloxacin at 4 MIC, than in the control plasma after incubation for 24 h (P <0.01). The colonies on the membranous structures completely disappeared in the case of plasma with 5% ZnO and 0.2% hinokitiol. The colony counts on membranous structures were lower in plasma with cefdinir at 4 MIC or levofloxacin at 4 MIC containing 5% ZnO than in plasma with cefdinir at 4 MIC or levofloxacin at 4 MIC only, (P <0.05). The MICs of hinokitiol against S. aureus strains peaked at an MIC distribution of 16-32 μg/ml. The peak shifted to below 1 μg/ml by adding 5% ZnO in agar plate method. The results suggest that the attachment of S. aureus cells to the coverslips is suppressed in the presence of 5% ZnO and that antistaphylococcal activities of cefdinir, levofloxacin and hinokitiol increase in the presence of 5% ZnO.
AB - We examined the attachment of Staphylococcus aureus to plastic tissue- culture coverslips after incubation for 24 h. The attachment to coverslips was weaker in rabbit plasma with 5% zinc oxide (ZnO) than in the control rabbit plasma without ZnO (P <0.01). Plasma coagulation by S. aureus strains was not detected in plasma with 5% ZnO after incubation for 24 h. The membranous structure (an immature biofilm) was formed on the coverslips by S. aureus cells in plasma after incubation for 24 h. The colony counts of S. aureus cells on the membranous structures were lower in plasma with 5% ZnO, plasma with 0.2% hinokitiol, plasma with 5% ZnO + 0.2% hinokitiol, plasma with cefdinir at 4 minimum inhibitory concentration (MIC) and plasma with levofloxacin at 4 MIC, than in the control plasma after incubation for 24 h (P <0.01). The colonies on the membranous structures completely disappeared in the case of plasma with 5% ZnO and 0.2% hinokitiol. The colony counts on membranous structures were lower in plasma with cefdinir at 4 MIC or levofloxacin at 4 MIC containing 5% ZnO than in plasma with cefdinir at 4 MIC or levofloxacin at 4 MIC only, (P <0.05). The MICs of hinokitiol against S. aureus strains peaked at an MIC distribution of 16-32 μg/ml. The peak shifted to below 1 μg/ml by adding 5% ZnO in agar plate method. The results suggest that the attachment of S. aureus cells to the coverslips is suppressed in the presence of 5% ZnO and that antistaphylococcal activities of cefdinir, levofloxacin and hinokitiol increase in the presence of 5% ZnO.
KW - Atopic dermatitis
KW - Attachment
KW - Staphylococcus aureus
KW - Zinc oxide
UR - http://www.scopus.com/inward/record.url?scp=0032076113&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032076113&partnerID=8YFLogxK
U2 - 10.1016/S0923-1811(97)00070-4
DO - 10.1016/S0923-1811(97)00070-4
M3 - Article
C2 - 9651831
AN - SCOPUS:0032076113
VL - 17
SP - 67
EP - 74
JO - Journal of Dermatological Science
JF - Journal of Dermatological Science
SN - 0923-1811
IS - 1
ER -