Effects of zinc ion on type A monoamine oxidase in monkey brain mitochondria

Toru Egashira; Fusako Takayama; Kumiko Sakai

doi:10.1016/S0006-2952(02)01608-8

Effects of zinc ion on type A monoamine oxidase in monkey brain mitochondria

Toru Egashira, Fusako Takayama, Kumiko Sakai

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The effects of ZnSO₄ on types A and B monoamine oxidase (MAO) isozymes in monkey brain mitochondria were investigated, in vitro. Type A MAO activity in monkey brain decreased to about 50% with 1μM ZnSO₄ using serotonin as a substrate, and this inhibition was proportional to the concentration of ZnSO₄. ZnSO₄ had no effect, however, on type B MAO activity in monkey brain using β-phenylethylamine as a substrate. The inhibition by ZnSO₄ of type A MAO activity was competitive and reversible. ZnSO₄ did not inhibit either type A or type B MAO activity in rat brain mitochondria. Almost similar results were also obtained when ZnCl₂ was used, in vitro. These results indicate that the inhibiting action of zinc ion differs depending on animal species and organ. Type A MAO in monkey brain mitochondria was highly sensitive to zinc ion, while type B activity was less sensitive.

Original language	English
Pages (from-to)	625-627
Number of pages	3
Journal	Biochemical Pharmacology
Volume	65
Issue number	4
DOIs	https://doi.org/10.1016/S0006-2952(02)01608-8
Publication status	Published - Feb 15 2003
Externally published	Yes

Keywords

Monkey brain
Rat brain
Type A MAO
Type A MAO inhibitor
Type B MAO
Zinc ion

ASJC Scopus subject areas

Biochemistry
Pharmacology

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10.1016/S0006-2952(02)01608-8

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title = "Effects of zinc ion on type A monoamine oxidase in monkey brain mitochondria",

abstract = "The effects of ZnSO4 on types A and B monoamine oxidase (MAO) isozymes in monkey brain mitochondria were investigated, in vitro. Type A MAO activity in monkey brain decreased to about 50% with 1μM ZnSO4 using serotonin as a substrate, and this inhibition was proportional to the concentration of ZnSO4. ZnSO4 had no effect, however, on type B MAO activity in monkey brain using β-phenylethylamine as a substrate. The inhibition by ZnSO4 of type A MAO activity was competitive and reversible. ZnSO4 did not inhibit either type A or type B MAO activity in rat brain mitochondria. Almost similar results were also obtained when ZnCl2 was used, in vitro. These results indicate that the inhibiting action of zinc ion differs depending on animal species and organ. Type A MAO in monkey brain mitochondria was highly sensitive to zinc ion, while type B activity was less sensitive.",

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author = "Toru Egashira and Fusako Takayama and Kumiko Sakai",

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doi = "10.1016/S0006-2952(02)01608-8",

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T1 - Effects of zinc ion on type A monoamine oxidase in monkey brain mitochondria

AU - Egashira, Toru

AU - Takayama, Fusako

AU - Sakai, Kumiko

PY - 2003/2/15

Y1 - 2003/2/15

N2 - The effects of ZnSO4 on types A and B monoamine oxidase (MAO) isozymes in monkey brain mitochondria were investigated, in vitro. Type A MAO activity in monkey brain decreased to about 50% with 1μM ZnSO4 using serotonin as a substrate, and this inhibition was proportional to the concentration of ZnSO4. ZnSO4 had no effect, however, on type B MAO activity in monkey brain using β-phenylethylamine as a substrate. The inhibition by ZnSO4 of type A MAO activity was competitive and reversible. ZnSO4 did not inhibit either type A or type B MAO activity in rat brain mitochondria. Almost similar results were also obtained when ZnCl2 was used, in vitro. These results indicate that the inhibiting action of zinc ion differs depending on animal species and organ. Type A MAO in monkey brain mitochondria was highly sensitive to zinc ion, while type B activity was less sensitive.

AB - The effects of ZnSO4 on types A and B monoamine oxidase (MAO) isozymes in monkey brain mitochondria were investigated, in vitro. Type A MAO activity in monkey brain decreased to about 50% with 1μM ZnSO4 using serotonin as a substrate, and this inhibition was proportional to the concentration of ZnSO4. ZnSO4 had no effect, however, on type B MAO activity in monkey brain using β-phenylethylamine as a substrate. The inhibition by ZnSO4 of type A MAO activity was competitive and reversible. ZnSO4 did not inhibit either type A or type B MAO activity in rat brain mitochondria. Almost similar results were also obtained when ZnCl2 was used, in vitro. These results indicate that the inhibiting action of zinc ion differs depending on animal species and organ. Type A MAO in monkey brain mitochondria was highly sensitive to zinc ion, while type B activity was less sensitive.

KW - Monkey brain

KW - Rat brain

KW - Type A MAO

KW - Type A MAO inhibitor

KW - Type B MAO

KW - Zinc ion

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JO - Biochemical Pharmacology

JF - Biochemical Pharmacology

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ER -

Effects of zinc ion on type A monoamine oxidase in monkey brain mitochondria

Abstract

Keywords

ASJC Scopus subject areas

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