Effects of vitamin C intake on gingival oxidative stress in rat periodontitis

Takaaki Tomofuji, Daisuke Ekuni, Toshihiro Sanbe, Koichiro Irie, Tetsuji Azuma, Takayuki Maruyama, Naofumi Tamaki, Jun Murakami, Susumu Kokeguchi, Tatsuo Yamamoto

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Increased levels of oxidative stress due to excessive production of reactive oxygen species are involved in the pathogenesis of periodontitis. Studies suggest a negative association between plasma vitamin C level and the severity of periodontitis. We hypothesized that increases in plasma vitamin C levels after vitamin C intake might clinically reduce gingival oxidative stress in a rat periodontitis model. A ligature was placed around rat mandibular molars for 4 weeks to induce periodontitis, and the rats were then given drinking water with or without 1 g/L vitamin C for 2 weeks after the ligature was removed. The periodontitis-induced rats showed a 149% increase in 8-hydroxydeoxyguanosine level and a 40% decrease in reduced:oxidized glutathione ratio in gingival tissue. Vitamin C intake induced a 175% increase in plasma vitamin C level, resulting in an improvement in the gingival 8-hydroxydeoxyguanosine level (decreased) and in the reduced:oxidized glutathione ratio (increased). Furthermore, in ligature-induced periodontitis lesions, gene expression encoding inflammation, including interleukin-1α and interleukin-1β, was more than twofold down-regulated by vitamin C intake. The results suggest that systemic administration of vitamin C could be clinically beneficial in improving periodontitis-induced oxidative stress by down-regulating inflammatory gene expression.

Original languageEnglish
Pages (from-to)163-168
Number of pages6
JournalFree Radical Biology and Medicine
Volume46
Issue number2
DOIs
Publication statusPublished - Jan 15 2009

Fingerprint

Oxidative stress
Periodontitis
Ascorbic Acid
Rats
Oxidative Stress
Ligation
Glutathione Disulfide
Interleukin-1
Plasmas
Gene expression
Glutathione
Gene Expression
Drinking Water
Reactive Oxygen Species
Association reactions
Tissue
Inflammation

Keywords

  • Animal studies
  • Free radicals
  • Oxidative stress
  • Periodontitis
  • Vitamin C

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

Cite this

Effects of vitamin C intake on gingival oxidative stress in rat periodontitis. / Tomofuji, Takaaki; Ekuni, Daisuke; Sanbe, Toshihiro; Irie, Koichiro; Azuma, Tetsuji; Maruyama, Takayuki; Tamaki, Naofumi; Murakami, Jun; Kokeguchi, Susumu; Yamamoto, Tatsuo.

In: Free Radical Biology and Medicine, Vol. 46, No. 2, 15.01.2009, p. 163-168.

Research output: Contribution to journalArticle

Tomofuji, Takaaki ; Ekuni, Daisuke ; Sanbe, Toshihiro ; Irie, Koichiro ; Azuma, Tetsuji ; Maruyama, Takayuki ; Tamaki, Naofumi ; Murakami, Jun ; Kokeguchi, Susumu ; Yamamoto, Tatsuo. / Effects of vitamin C intake on gingival oxidative stress in rat periodontitis. In: Free Radical Biology and Medicine. 2009 ; Vol. 46, No. 2. pp. 163-168.
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