Effects of vasopressin and involvement of receptor subtypes in the rat central amygdaloid nucleus in vitro

Yun Fei Lu; A. Moriwaki; K. Tomizawa; H. Onuma; X. H. Cai; H. Matsui

doi:10.1016/S0006-8993(97)00655-0

Effects of vasopressin and involvement of receptor subtypes in the rat central amygdaloid nucleus in vitro

Yun Fei Lu, A. Moriwaki, K. Tomizawa, H. Onuma, X. H. Cai, H. Matsui

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Effects of arginine-vasopressin (AVP) on neurons in the central amygdaloid nucleus (ACe) were investigated with rat brain slice preparations using extracellular recording methods. Of 160 ACe neurons tested, 70 cells (44%) were excited and 9 cells (6%) were inhibited by bath application of AVP at 3 X 10^-7 M. The excitatory effects of AVP were dose-dependent and the threshold concentration was approximately 10^-10 to 10^-9 M. The excitatory effects of AVP persisted under blockade of synaptic transmission by perfusing with Ca²⁺-free and high-Mg²⁺ medium, whereas the inhibitory effects were abolished by synaptic blockade. AVP-induced effects were mimicked by a V₁-receptor agonist and completely blocked by a selective V₁- antagonist. V₂-agonist produced no effects on ACe neurons and V₂-antagonist had no effect on AVP-induced excitation. These results showed that the excitatory effect of AVP on ACe neurons was produced by a direct action through the V₁-receptors, whereas the inhibitory response of ACe neurons to AVP seemed to be produced by an indirect action. The results of this study suggest that AVP is involved in the amygdala-relevant functions as a neurotransmitter or a neuromodulator.

Original language	English
Pages (from-to)	266-272
Number of pages	7
Journal	Brain Research
Volume	768
Issue number	1-2
DOIs	https://doi.org/10.1016/S0006-8993(97)00655-0
Publication status	Published - Sep 12 1997

Keywords

Amygdala
Brain slice
Central amygdaloid nucleus
Neuron activity
Vasopressin

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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abstract = "Effects of arginine-vasopressin (AVP) on neurons in the central amygdaloid nucleus (ACe) were investigated with rat brain slice preparations using extracellular recording methods. Of 160 ACe neurons tested, 70 cells (44%) were excited and 9 cells (6%) were inhibited by bath application of AVP at 3 X 10-7 M. The excitatory effects of AVP were dose-dependent and the threshold concentration was approximately 10-10 to 10-9 M. The excitatory effects of AVP persisted under blockade of synaptic transmission by perfusing with Ca2+-free and high-Mg2+ medium, whereas the inhibitory effects were abolished by synaptic blockade. AVP-induced effects were mimicked by a V1-receptor agonist and completely blocked by a selective V1- antagonist. V2-agonist produced no effects on ACe neurons and V2-antagonist had no effect on AVP-induced excitation. These results showed that the excitatory effect of AVP on ACe neurons was produced by a direct action through the V1-receptors, whereas the inhibitory response of ACe neurons to AVP seemed to be produced by an indirect action. The results of this study suggest that AVP is involved in the amygdala-relevant functions as a neurotransmitter or a neuromodulator.",

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AU - Lu, Yun Fei

AU - Moriwaki, A.

AU - Tomizawa, K.

AU - Onuma, H.

AU - Cai, X. H.

AU - Matsui, H.

PY - 1997/9/12

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AB - Effects of arginine-vasopressin (AVP) on neurons in the central amygdaloid nucleus (ACe) were investigated with rat brain slice preparations using extracellular recording methods. Of 160 ACe neurons tested, 70 cells (44%) were excited and 9 cells (6%) were inhibited by bath application of AVP at 3 X 10-7 M. The excitatory effects of AVP were dose-dependent and the threshold concentration was approximately 10-10 to 10-9 M. The excitatory effects of AVP persisted under blockade of synaptic transmission by perfusing with Ca2+-free and high-Mg2+ medium, whereas the inhibitory effects were abolished by synaptic blockade. AVP-induced effects were mimicked by a V1-receptor agonist and completely blocked by a selective V1- antagonist. V2-agonist produced no effects on ACe neurons and V2-antagonist had no effect on AVP-induced excitation. These results showed that the excitatory effect of AVP on ACe neurons was produced by a direct action through the V1-receptors, whereas the inhibitory response of ACe neurons to AVP seemed to be produced by an indirect action. The results of this study suggest that AVP is involved in the amygdala-relevant functions as a neurotransmitter or a neuromodulator.

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Effects of vasopressin and involvement of receptor subtypes in the rat central amygdaloid nucleus in vitro

Abstract

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