Effects of various salts and irradiation with UV light on the attachment of Staphylococcus aureus strains

Hisanori Akiyama, Osamu Yamasaki, Hiroko Kanzaki, Joji Tada, Jirô Arata

Research output: Contribution to journalArticle

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Abstract

We investigated the attachment of Staphylococcus aureus isolated from atopic dermatitis lesions to plastic tissue-culture coverslips. We found that attachment was weaker in (rabbit) plasma with 5 or 10% NaCl and in plasma with 5 or 10% sea salts than in the control plasma after incubation for 2 h (P <0.01). The attachment was weaker still in plasma with 10% NaCl or 10% sea salts than in the control plasma after incubation for 24 h (P <0.01). Plasma coagulation of four S. aureus strains isolated from atopic dermatitis lesions was not detected in plasma with 10% NaCl (pH 5.6) or 10% sea salts (containing 0.372% Mg2+) after incubation for 12, 24, 36 and 60 h. The attachment of S. aureus strain cells to the coverslip in plasma was weaker after irradiation with UVA at 25 or 50 J/cm2 (P <0.01) and UVB at 0.5 J/cm2 (P <0.05) both of which are covered by a black cloth, than without irradiation after incubation for 24 h. Plasma coagulation was not detected after irradiation with UVA at 25 or 50 J/cm2 with a black cloth cover (temperature reached 50°C), but was detected after UVA irradiation at the same doses combined with cooling (temperature reached 22°C), after incubation for 24 h. The results suggest that the attachment of S. aureus cells isolated from atopic dermatitis lesions to the coverslip is suppressed in the presence of 10% salts and irradiation with UVA and UVB, and that plasma coagulation of S. aureus cells isolated from atopic dermatitis lesions is suppressed in the presence of 10% salts, irradiation with UVA, and heating.

Original languageEnglish
Pages (from-to)216-225
Number of pages10
JournalJournal of Dermatological Science
Volume16
Issue number3
DOIs
Publication statusPublished - Mar 1998

Fingerprint

Ultraviolet Rays
Ultraviolet radiation
Staphylococcus aureus
Salts
Irradiation
Plasmas
Atopic Dermatitis
Coagulation
Oceans and Seas
Tissue culture
Temperature
Heating
Dosimetry
Rabbits
Cooling

Keywords

  • Atopic dermatitis
  • Attachment
  • Salts
  • Staphylococcus aureus
  • UV

ASJC Scopus subject areas

  • Dermatology

Cite this

Effects of various salts and irradiation with UV light on the attachment of Staphylococcus aureus strains. / Akiyama, Hisanori; Yamasaki, Osamu; Kanzaki, Hiroko; Tada, Joji; Arata, Jirô.

In: Journal of Dermatological Science, Vol. 16, No. 3, 03.1998, p. 216-225.

Research output: Contribution to journalArticle

Akiyama, Hisanori ; Yamasaki, Osamu ; Kanzaki, Hiroko ; Tada, Joji ; Arata, Jirô. / Effects of various salts and irradiation with UV light on the attachment of Staphylococcus aureus strains. In: Journal of Dermatological Science. 1998 ; Vol. 16, No. 3. pp. 216-225.
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abstract = "We investigated the attachment of Staphylococcus aureus isolated from atopic dermatitis lesions to plastic tissue-culture coverslips. We found that attachment was weaker in (rabbit) plasma with 5 or 10{\%} NaCl and in plasma with 5 or 10{\%} sea salts than in the control plasma after incubation for 2 h (P <0.01). The attachment was weaker still in plasma with 10{\%} NaCl or 10{\%} sea salts than in the control plasma after incubation for 24 h (P <0.01). Plasma coagulation of four S. aureus strains isolated from atopic dermatitis lesions was not detected in plasma with 10{\%} NaCl (pH 5.6) or 10{\%} sea salts (containing 0.372{\%} Mg2+) after incubation for 12, 24, 36 and 60 h. The attachment of S. aureus strain cells to the coverslip in plasma was weaker after irradiation with UVA at 25 or 50 J/cm2 (P <0.01) and UVB at 0.5 J/cm2 (P <0.05) both of which are covered by a black cloth, than without irradiation after incubation for 24 h. Plasma coagulation was not detected after irradiation with UVA at 25 or 50 J/cm2 with a black cloth cover (temperature reached 50°C), but was detected after UVA irradiation at the same doses combined with cooling (temperature reached 22°C), after incubation for 24 h. The results suggest that the attachment of S. aureus cells isolated from atopic dermatitis lesions to the coverslip is suppressed in the presence of 10{\%} salts and irradiation with UVA and UVB, and that plasma coagulation of S. aureus cells isolated from atopic dermatitis lesions is suppressed in the presence of 10{\%} salts, irradiation with UVA, and heating.",
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