Effects of UVB and UVC irradiation on cariogenic bacteria in vitro

Shigeki Uchinuma; Yasushi Shimada; Khairul Matin; Keiichi Hosaka; Masahiro Yoshiyama; Yasunori Sumi; Junji Tagami

doi:10.1007/s10103-018-2685-4

Effects of UVB and UVC irradiation on cariogenic bacteria in vitro

Shigeki Uchinuma, Yasushi Shimada, Khairul Matin, Keiichi Hosaka, Masahiro Yoshiyama, Yasunori Sumi, Junji Tagami

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The aim of this study was to evaluate the bactericidal effect of ultraviolet (UV) light at 265-nm (UVC) and 310-nm (UVB) wavelengths from a newly developed UV light-emitting diode (LED) device against cariogenic bacteria in vitro. Suspensions of Streptococcus mutans (S. mutans) and Streptococcus sobrinus were directly irradiated by UVB or UVC for 2.5 min or 5 min. Numbers of colonies were counted and calculated as colony forming units (CFU) per milliliter. Fluorescence microscopy (FM) and optical density measurements at 490 nm (OD₄₉₀) were also taken after irradiation. In addition, the bactericidal effects of irradiation against S. mutans under 0.5 mm-thick dentin were compared using culture tests and OD₄₉₀ measurements. Direct UV-LED irradiation with both UVB and UVC showed strong bactericidal effects. UVB showed superior bactericidal effect through 0.5-mm-thick dentin than did UVC, especially after demineralization. These results suggest that UVB irradiation could be utilized for the prevention and management of dental caries.

Original language	English
Pages (from-to)	981-989
Number of pages	9
Journal	Lasers in Medical Science
Volume	34
Issue number	5
DOIs	https://doi.org/10.1007/s10103-018-2685-4
Publication status	Published - Jul 1 2019

Keywords

Demineralized dentin
Streptococcus mutans
Streptococcus sobrinus
UV-LED
UVB
UVC

ASJC Scopus subject areas

Surgery
Dermatology

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10.1007/s10103-018-2685-4

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title = "Effects of UVB and UVC irradiation on cariogenic bacteria in vitro",

abstract = "The aim of this study was to evaluate the bactericidal effect of ultraviolet (UV) light at 265-nm (UVC) and 310-nm (UVB) wavelengths from a newly developed UV light-emitting diode (LED) device against cariogenic bacteria in vitro. Suspensions of Streptococcus mutans (S. mutans) and Streptococcus sobrinus were directly irradiated by UVB or UVC for 2.5 min or 5 min. Numbers of colonies were counted and calculated as colony forming units (CFU) per milliliter. Fluorescence microscopy (FM) and optical density measurements at 490 nm (OD490) were also taken after irradiation. In addition, the bactericidal effects of irradiation against S. mutans under 0.5 mm-thick dentin were compared using culture tests and OD490 measurements. Direct UV-LED irradiation with both UVB and UVC showed strong bactericidal effects. UVB showed superior bactericidal effect through 0.5-mm-thick dentin than did UVC, especially after demineralization. These results suggest that UVB irradiation could be utilized for the prevention and management of dental caries.",

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author = "Shigeki Uchinuma and Yasushi Shimada and Khairul Matin and Keiichi Hosaka and Masahiro Yoshiyama and Yasunori Sumi and Junji Tagami",

note = "Funding Information: Funding information This research project was supported by the Research Grant for Longevity Science (29-3) from the Japanese Ministry of Health, Labor and Welfare, and by a Grant-in-Aid for Scientific Research (16 K11544) from the Japan Society for the Promotion of Science (JSPS). Publisher Copyright: {\textcopyright} 2018, Springer-Verlag London Ltd., part of Springer Nature.",

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AU - Uchinuma, Shigeki

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AU - Yoshiyama, Masahiro

AU - Sumi, Yasunori

AU - Tagami, Junji

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N2 - The aim of this study was to evaluate the bactericidal effect of ultraviolet (UV) light at 265-nm (UVC) and 310-nm (UVB) wavelengths from a newly developed UV light-emitting diode (LED) device against cariogenic bacteria in vitro. Suspensions of Streptococcus mutans (S. mutans) and Streptococcus sobrinus were directly irradiated by UVB or UVC for 2.5 min or 5 min. Numbers of colonies were counted and calculated as colony forming units (CFU) per milliliter. Fluorescence microscopy (FM) and optical density measurements at 490 nm (OD490) were also taken after irradiation. In addition, the bactericidal effects of irradiation against S. mutans under 0.5 mm-thick dentin were compared using culture tests and OD490 measurements. Direct UV-LED irradiation with both UVB and UVC showed strong bactericidal effects. UVB showed superior bactericidal effect through 0.5-mm-thick dentin than did UVC, especially after demineralization. These results suggest that UVB irradiation could be utilized for the prevention and management of dental caries.

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Effects of UVB and UVC irradiation on cariogenic bacteria in vitro

Abstract

Keywords

ASJC Scopus subject areas

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