Effects of UVB and UVC irradiation on cariogenic bacteria in vitro

Shigeki Uchinuma, Yasushi Shimada, Khairul Matin, Keiichi Hosaka, Masahiro Yoshiyama, Yasunori Sumi, Junji Tagami

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The aim of this study was to evaluate the bactericidal effect of ultraviolet (UV) light at 265-nm (UVC) and 310-nm (UVB) wavelengths from a newly developed UV light-emitting diode (LED) device against cariogenic bacteria in vitro. Suspensions of Streptococcus mutans (S. mutans) and Streptococcus sobrinus were directly irradiated by UVB or UVC for 2.5 min or 5 min. Numbers of colonies were counted and calculated as colony forming units (CFU) per milliliter. Fluorescence microscopy (FM) and optical density measurements at 490 nm (OD490) were also taken after irradiation. In addition, the bactericidal effects of irradiation against S. mutans under 0.5 mm-thick dentin were compared using culture tests and OD490 measurements. Direct UV-LED irradiation with both UVB and UVC showed strong bactericidal effects. UVB showed superior bactericidal effect through 0.5-mm-thick dentin than did UVC, especially after demineralization. These results suggest that UVB irradiation could be utilized for the prevention and management of dental caries.

Original languageEnglish
Pages (from-to)981-989
Number of pages9
JournalLasers in Medical Science
Volume34
Issue number5
DOIs
Publication statusPublished - Jul 1 2019

Keywords

  • Demineralized dentin
  • Streptococcus mutans
  • Streptococcus sobrinus
  • UV-LED
  • UVB
  • UVC

ASJC Scopus subject areas

  • Surgery
  • Dermatology

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