Effects of tumor necrosis factor α on proliferation and expression of differentiated phenotypes in rabbit costal chondrocytes in culture

Motomi Enomoto, Hai Ou Pan, Akihiro Kinoshita, Yasutaka Yutani, Fujio Suzuki, Masaharu Takigawa

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Tumor necrosis factor α (TNFα) decreased the synthesis of glycosaminoglycan (GAG) in rabbit costal chondrocytes in culture, but did not stimulate the release of GAG from cell layers. Like chondrocytes cultured in control medium, chondrocytes cultured in the presence of TNFα produced putative "cartilage-specific" proteoglycans identified by density gradient centrifugation under dissociative conditions. Although TNFα decreased the synthesis of the proteoglycans, it did not change their monomeric size, which is a marker of cartilage phenotypes. Moreover, TNFα did not affect the responsiveness to parathyroid hormone, insulin-like growth factor I, or transforming growth factor β, which is known to stimulate GAG synthesis in cultured chondrocytes. TNFα decreased the alkaline phosphatase activity in the chondrocytes dose dependently. On the other hand, it stimulated their DNA synthesis slightly, but significantly. The stimulatory effect of TNFα on DNA synthesis was potentiated by fibroblast growth factor, epidermal growth factor, and fetal bovine serum. These findings suggest that in the presence of hormones and growth factors, TNFα promotes the proliferation of chondrocytes while suppressing their further differentiation at the stage of synthesis of cartilage-specific proteoglycans.

Original languageEnglish
Pages (from-to)145-151
Number of pages7
JournalCalcified Tissue International
Volume47
Issue number3
DOIs
Publication statusPublished - Sep 1 1990
Externally publishedYes

Keywords

  • Alkaline phosphatase
  • Chondrocytes
  • Proliferation
  • Proteoglycan
  • Tumor necrosis factor α

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine
  • Endocrinology

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