Effects of tumor necrosis factor-α and nitric oxide on prostaglandins secretion by the bovine oviduct differ in the isthmus and ampulla and depend on the phase of the estrous cycle

Marta J. Siemieniuch, Izabela Woclawek-Potocka, Katarzyna Deptula, Kiyoshi Okuda, Dariusz J. Skarzynski

Research output: Contribution to journalArticle

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Abstract

To determine the possible roles of tumor necrosis factor-α (TNFα) and nitric oxide (NO) in the bovine oviduct, ampulla and isthmus collected during the estrous cycle were exposed for 18 h to TNFα, NO donor (NONOate), NO synthase inhibitors (L-NOARG, L-NAME and AMT) and oxytocin (OT) as a positive control. Prostaglandins (PGs) and NO2/NO3 in conditioned media were measured. TNFα stimulated PGF secretion on Day 0 (onset of estrus = Day 0) and Days 2-3, in both the ampulla and isthmus, but on Days 18-20 only in ampulla. TNFα increased PGE 2 secretion in both fragments in each phase. NONOate did not affect PGF secretion on Days 18-20, whereas this NO donor stimulated PGF secretion in both fragments on Day 0 and Days 2-3. TNFα increased NO2/NO3 production in every examined phase in the ampulla and on Days 2-3 in the isthmus. L-NAME lowered NO 2/NO3 production regardless of phase or fragment. L-NOARG and AMT lowered NO2/NO3 production in both fragments on Day 0 and Days 2-3. The possible role of TNFα, NO or PGs on the oviductal contractility during the early-luteal phase was also examined. Neither TNFα nor NONOate influenced contractility in either fragment. Although PGF stimulated the contraction in both fragments, PGE 2 decreased it. When taken together, TNFα seems to play some role as a modulator of PGF and PGE2 production and for transferring the embryo from the oviduct to the uterus by stimulating NO production in the bovine oviduct.

Original languageEnglish
Pages (from-to)1056-1066
Number of pages11
JournalExperimental Biology and Medicine
Volume234
Issue number9
DOIs
Publication statusPublished - Sep 2009

Fingerprint

Oviducts
Estrous Cycle
Prostaglandins
Nitric Oxide
Tumor Necrosis Factor-alpha
Dinoprost
Nitric Oxide Donors
Nitroarginine
NG-Nitroarginine Methyl Ester
Prostaglandins E
Luteal Phase
Estrus
Oxytocin
Conditioned Culture Medium
Dinoprostone
Nitric Oxide Synthase
Modulators
Uterus
Embryonic Structures

Keywords

  • Cattle
  • Nitric oxide
  • Oviduct
  • Prostaglandins
  • TNFα

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Effects of tumor necrosis factor-α and nitric oxide on prostaglandins secretion by the bovine oviduct differ in the isthmus and ampulla and depend on the phase of the estrous cycle. / Siemieniuch, Marta J.; Woclawek-Potocka, Izabela; Deptula, Katarzyna; Okuda, Kiyoshi; Skarzynski, Dariusz J.

In: Experimental Biology and Medicine, Vol. 234, No. 9, 09.2009, p. 1056-1066.

Research output: Contribution to journalArticle

Siemieniuch, Marta J. ; Woclawek-Potocka, Izabela ; Deptula, Katarzyna ; Okuda, Kiyoshi ; Skarzynski, Dariusz J. / Effects of tumor necrosis factor-α and nitric oxide on prostaglandins secretion by the bovine oviduct differ in the isthmus and ampulla and depend on the phase of the estrous cycle. In: Experimental Biology and Medicine. 2009 ; Vol. 234, No. 9. pp. 1056-1066.
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AU - Okuda, Kiyoshi

AU - Skarzynski, Dariusz J.

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