Effects of trimethylsilylation of copper(II)-phthalocyanine sulfonyl-aminopropyl silica gels on the separation of π-electron-rich compounds by high-performance liquid chromatography

A. Iwado, M. Mifune, Y. Mori, M. Onoda, T. Kanai, N. Motohashi, J. Haginaka, Y. Saito

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

As an attempt to elucidate the factor(s) responsible for the poor performance of a copper(II)-phthalocyanine aminopropyl-silica gels (Cu-PCS D ) column for HPLC, the silanol and/or amino groups remaining on Cu-PCS D were endcapped with trimethylchlorosilane (TMCS) or N-trimethylsilylimidazole (TMSI). The trimethylsilylated Cu-PCS D s (Cu-PCS D -TMCS and -TMSI) were investigated concerning their performance as an HPLC-stationary phase in the separation of πelectronrich polyaromatic hydrocarbons (PAHs), such as mutagenic anthracene and pyrene. As a result, trimethylsilylation with TMSI, which reacts only with silanol-groups, was not effective to improve the column efficiency. In contrast, trimethylsilylation by TMCS, which reacts with both the silanol- and amino-groups, improved the theoretical plate numbers (N) for PAHs separation with the Cu-PCS D column, indicating that the low N values on the Cu-PCS D column were caused by electrostatic interactions between PAHs and the remaining amino-groups on Cu-PCS D . Furthermore, the retention data of mutagenic heterocyclic amines (HCAs) indicated that the remaining amino groups interact with the polar groups of HCAs.

Original languageEnglish
Pages (from-to)301-305
Number of pages5
JournalAnalytical Sciences
Volume17
Issue number2
DOIs
Publication statusPublished - Jan 1 2001

ASJC Scopus subject areas

  • Analytical Chemistry

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