Effects of transforming growth factor-β1 on formation and activation of osteoclasts

Hiroshi Kamioka, Keishi Ishikawa, Kayo Tanaka, Takuya Sato, Ken Ichi Tezuka, Kenji Hiura, Koji Sumitani, Yoshiyuki Hakeda, Terushige Kawata, Masayoshi Kumegawa

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Transforming growth factor-β1 (TGF-β1) has biological functions in various types of cells. However, its roles in the regulation of osteoclast formation and function are unclear. To examine them, we employed a culture system in which unfractionated cells obtained from long bones of 13-day-old mice were cultured on a dentine slice. We found that TGF-β1 has a potent inhibitory effect on osteoclastic bone resorption at a dose of 0.2-5 ng/ml. By electron microscopy the osteoclasts appeared to have fewer mitochondria and ruffled borders than those in control cultures. But in the presence of 1,25-dihydroxyvitamin D3, [1,25-(OH)2D3], TGF-β1 at a dose of 0.2-1 ng/ml stimulated the formation of osteoclasts from unfractionated bone cell cultures in which preexistent osteoclasts had degenerated. Thus, using stromal cell-free he-mopoietic blast cells, we examined the direct action of TGF-β1 on osteoclast precursors. Although TGF-β1 inhibited tartrate-resistant acid phosphatase-positive (TRAP) multinucleate cell (MNC) formation induced by 1,25-(OH)2D3, the conditioned medium (CM) of TGF-β1-treated MC3T3-E1 cells stimulated such formation. These results suggest that TGF-β1 inhibits osteoclastic bone resorption but stimulates osteoclast formation via the action of factor(s) produced by TGF-β1-treated osteoblasts in the presence of 1,25-(OH)2D3.

Original languageEnglish
Pages (from-to)3-9
Number of pages7
JournalJournal of Bone and Mineral Metabolism
Volume13
Issue number1
DOIs
Publication statusPublished - Mar 1995
Externally publishedYes

Fingerprint

Transforming Growth Factors
Osteoclasts
Bone Resorption
Bone and Bones
Calcitriol
Dentin
Stromal Cells
Conditioned Culture Medium
Osteoblasts
Electron Microscopy
Mitochondria
Cell Culture Techniques

Keywords

  • bone resorption
  • osteoclast
  • TGF-β

ASJC Scopus subject areas

  • Endocrinology

Cite this

Effects of transforming growth factor-β1 on formation and activation of osteoclasts. / Kamioka, Hiroshi; Ishikawa, Keishi; Tanaka, Kayo; Sato, Takuya; Tezuka, Ken Ichi; Hiura, Kenji; Sumitani, Koji; Hakeda, Yoshiyuki; Kawata, Terushige; Kumegawa, Masayoshi.

In: Journal of Bone and Mineral Metabolism, Vol. 13, No. 1, 03.1995, p. 3-9.

Research output: Contribution to journalArticle

Kamioka, H, Ishikawa, K, Tanaka, K, Sato, T, Tezuka, KI, Hiura, K, Sumitani, K, Hakeda, Y, Kawata, T & Kumegawa, M 1995, 'Effects of transforming growth factor-β1 on formation and activation of osteoclasts', Journal of Bone and Mineral Metabolism, vol. 13, no. 1, pp. 3-9. https://doi.org/10.1007/BF01771798
Kamioka, Hiroshi ; Ishikawa, Keishi ; Tanaka, Kayo ; Sato, Takuya ; Tezuka, Ken Ichi ; Hiura, Kenji ; Sumitani, Koji ; Hakeda, Yoshiyuki ; Kawata, Terushige ; Kumegawa, Masayoshi. / Effects of transforming growth factor-β1 on formation and activation of osteoclasts. In: Journal of Bone and Mineral Metabolism. 1995 ; Vol. 13, No. 1. pp. 3-9.
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