Effects of toothbrushing frequency on proliferation of gingival cells and collagen synthesis

Tatsuo Yamamoto, Takaaki Tomofuji, Daisuke Ekuni, Tomonori Sakamoto, Masazumi Horiuchi, Tatsuo Watanabe

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Mechanical stimulation by toothbrushing enhances proliferation of fibroblasts and junctional epithelium (JE). These changes in gingiva may depend on the interval between toothbrushing. The effects of toothbrushing frequency on proliferation of gingival fibroblasts and basal cells of JE were evaluated. Methods: Twelve mongrel dogs were used. Each tooth was brushed for 20 s at 1.96 N. The subepithelial connective tissue of JE was examined for proliferating cell nuclear antigen (PCNA)-positive fibroblasts and procollagen type-I C-peptide (PIP)-positive fibroblasts. JE was examined for PCNA-positive basal cells. Results: Gingiva that received brushing twice a day showed increases in the density of fibroblasts and ratio of PCNA-positive fibroblasts to total fibroblasts at 4 weeks. The ratio of PIP-positive fibroblasts increased at 8 weeks in gingiva brushed twice a day and once a day. PCNA-positive basal cell ratio increased at 4 weeks in gingiva brushed twice a day and once a day. Conclusions: A high frequency of brushing was associated with increased numbers of PCNA-positive fibroblasts, PIP-positive fibroblasts and PCNA-positive basal cells. Gingival cell proliferation increased and reached a plateau earlier in gingiva brushed twice a day than in gingiva brushed once a day.

Original languageEnglish
Pages (from-to)40-44
Number of pages5
JournalJournal of Clinical Periodontology
Volume31
Issue number1
DOIs
Publication statusPublished - Jan 2004

Fingerprint

Toothbrushing
Collagen
Fibroblasts
Cell Proliferation
Proliferating Cell Nuclear Antigen
Gingiva
Epithelial Attachment
C-Peptide
Collagen Type I
Connective Tissue
Tooth
Dogs

Keywords

  • Animal studies
  • Gingivitis
  • Procollagen type I
  • Proliferating cell nuclear antigen
  • Toothbrushing

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Effects of toothbrushing frequency on proliferation of gingival cells and collagen synthesis. / Yamamoto, Tatsuo; Tomofuji, Takaaki; Ekuni, Daisuke; Sakamoto, Tomonori; Horiuchi, Masazumi; Watanabe, Tatsuo.

In: Journal of Clinical Periodontology, Vol. 31, No. 1, 01.2004, p. 40-44.

Research output: Contribution to journalArticle

Yamamoto, Tatsuo ; Tomofuji, Takaaki ; Ekuni, Daisuke ; Sakamoto, Tomonori ; Horiuchi, Masazumi ; Watanabe, Tatsuo. / Effects of toothbrushing frequency on proliferation of gingival cells and collagen synthesis. In: Journal of Clinical Periodontology. 2004 ; Vol. 31, No. 1. pp. 40-44.
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