Effects of storage and passage of bovine luteal endothelial cells on endothelin-1 and prostaglandin F2α production

Tomas J. Acosta, Shin Yoshioka, Junichi Komiyama, Seung Hyung Lee, Anna T. Grazul-Bilska, Dariusz J. Skarzynski, Kiyoshi Okuda

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

To establish a storage system for isolated bovine luteal endothelial cells (LECs), we investigated the basal and tumor necrosis factor (TNF) α-stimulated production of endothelin-1 (ET-1) and prostaglandin (PG) F2α in unfrozen and frozen-thawed LECs until passage 10. LECs were obtained from developing corpora lutea (CL; days 5-7 of the estrous cycle) using enzymatic digestion and magnetic beads coated with lectin BS-1. The LECs were frozen at -80 C or further cultured and/ or passaged until passage 10 in DMEM/Ham's F-12 supplemented with 10% calf serum. The hormonal productions of unfrozen and frozen/thawed LECs were compared through passages 2-10. When both the unfrozen and frozen/thawed cells reached confluence, the culture medium was replaced with fresh medium containing 0.1% bovine serum albumin (BSA), and the cells were incubated with TNFα (50 ng/ml) for 12 h. The basal productions of ET-1 and PGF2α by the unfrozen and frozen/thawed LECs were similar at passage 2. The basal production of PGF2α by LECs was not altered by passage and storage at -80 C, whereas the basal production of ET-1 decreased from passage 2 and 3 to passage 4 in the unfrozen LECs and from passage 2 to passage 3 in the frozen/thawed LECs. However, production of ET-1 by the unfrozen and frozen/thawed LECs was similar between passages 4-10 and passages 3-10, respectively. Exposure of LECs to TNFα increased (P

Original languageEnglish
Pages (from-to)473-480
Number of pages8
JournalJournal of Reproduction and Development
Volume53
Issue number3
DOIs
Publication statusPublished - Jun 2007

Fingerprint

Luteal Cells
luteal cells
endothelins
Dinoprost
Endothelin-1
prostaglandins
endothelial cells
Endothelial Cells
cattle
tumor necrosis factors
Tumor Necrosis Factor-alpha
Estrous Cycle
Corpus Luteum
ham
bovine serum albumin
Bovine Serum Albumin
corpus luteum
Lectins
estrous cycle
lectins

Keywords

  • Bovine
  • Corpus luteum
  • Cytokines
  • Endothelin-1
  • Endothelium
  • Prostaglandins

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Reproductive Medicine

Cite this

Acosta, T. J., Yoshioka, S., Komiyama, J., Lee, S. H., Grazul-Bilska, A. T., Skarzynski, D. J., & Okuda, K. (2007). Effects of storage and passage of bovine luteal endothelial cells on endothelin-1 and prostaglandin F2α production. Journal of Reproduction and Development, 53(3), 473-480. https://doi.org/10.1262/jrd.18142

Effects of storage and passage of bovine luteal endothelial cells on endothelin-1 and prostaglandin F2α production. / Acosta, Tomas J.; Yoshioka, Shin; Komiyama, Junichi; Lee, Seung Hyung; Grazul-Bilska, Anna T.; Skarzynski, Dariusz J.; Okuda, Kiyoshi.

In: Journal of Reproduction and Development, Vol. 53, No. 3, 06.2007, p. 473-480.

Research output: Contribution to journalArticle

Acosta, TJ, Yoshioka, S, Komiyama, J, Lee, SH, Grazul-Bilska, AT, Skarzynski, DJ & Okuda, K 2007, 'Effects of storage and passage of bovine luteal endothelial cells on endothelin-1 and prostaglandin F2α production', Journal of Reproduction and Development, vol. 53, no. 3, pp. 473-480. https://doi.org/10.1262/jrd.18142
Acosta, Tomas J. ; Yoshioka, Shin ; Komiyama, Junichi ; Lee, Seung Hyung ; Grazul-Bilska, Anna T. ; Skarzynski, Dariusz J. ; Okuda, Kiyoshi. / Effects of storage and passage of bovine luteal endothelial cells on endothelin-1 and prostaglandin F2α production. In: Journal of Reproduction and Development. 2007 ; Vol. 53, No. 3. pp. 473-480.
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