Effects of repeated injection of cyclosporin A on pentylenetetrazol-induced convulsion and cyclophilin mRNA levels in rat brain

Masato Asanuma, Norio Ogawa, Sakiko Nishibayashi, Yoichi Kondo, Akitane Mori

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

To investigate the relationship between the immune system and convulsions in an animal model, we examined the effects of repeated administration with the immunosuppressant cyclosporin A on pentylenetetrazol (PTZ)-induced convulsions and the changes in the mRNA expression of its binding protein cyclophilin in the rat brain. The consecutive administration of cyclosporin A (5 mg/kg s.c., 14 days) significantly aggravated the severity of convulsions induced with PTZ 75 mg/kg i.p. Furthermore, it down-regulated the levels of cyclophilin mRNA in several brain regions and inhibited the PTZ-induced increase of hippocampal cyclophilin mRNA. Compared with the group without PTZ pretreatment or the group treated with chronic vehicle administration after the PTZ-preinjection, chronic cyclosporin A administration after the initial injection of PTZ apparently aggravated convulsions after the second PTZ injection. Interestingly, the increase in hippocampal cyclophilin mRNA observed after a single PTZ injection was not found after the second PTZ injection in the group with PTZ pretreatment. Therefore, these findings suggest that cyclosporin A administered peripherally can affect the central nervous system, and that an immune response associated with the first convulsive episode plays a key role in severity during subsequent attacks.

Original languageEnglish
Pages (from-to)101-105
Number of pages5
JournalNeurochemical Research
Volume20
Issue number1
DOIs
Publication statusPublished - Jan 1995

Fingerprint

Cyclophilins
Pentylenetetrazole
Cyclosporine
Rats
Brain
Seizures
Messenger RNA
Injections
Immune system
Neurology
Immunosuppressive Agents
Immune System
Carrier Proteins
Animals
Central Nervous System
Animal Models

Keywords

  • convulsion
  • cyclophilin
  • Cyclosporin A
  • pentylenetetrazol
  • rat brain
  • seizures

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

Effects of repeated injection of cyclosporin A on pentylenetetrazol-induced convulsion and cyclophilin mRNA levels in rat brain. / Asanuma, Masato; Ogawa, Norio; Nishibayashi, Sakiko; Kondo, Yoichi; Mori, Akitane.

In: Neurochemical Research, Vol. 20, No. 1, 01.1995, p. 101-105.

Research output: Contribution to journalArticle

Asanuma, Masato ; Ogawa, Norio ; Nishibayashi, Sakiko ; Kondo, Yoichi ; Mori, Akitane. / Effects of repeated injection of cyclosporin A on pentylenetetrazol-induced convulsion and cyclophilin mRNA levels in rat brain. In: Neurochemical Research. 1995 ; Vol. 20, No. 1. pp. 101-105.
@article{f539ef5495594047a7b19f98f5aa3498,
title = "Effects of repeated injection of cyclosporin A on pentylenetetrazol-induced convulsion and cyclophilin mRNA levels in rat brain",
abstract = "To investigate the relationship between the immune system and convulsions in an animal model, we examined the effects of repeated administration with the immunosuppressant cyclosporin A on pentylenetetrazol (PTZ)-induced convulsions and the changes in the mRNA expression of its binding protein cyclophilin in the rat brain. The consecutive administration of cyclosporin A (5 mg/kg s.c., 14 days) significantly aggravated the severity of convulsions induced with PTZ 75 mg/kg i.p. Furthermore, it down-regulated the levels of cyclophilin mRNA in several brain regions and inhibited the PTZ-induced increase of hippocampal cyclophilin mRNA. Compared with the group without PTZ pretreatment or the group treated with chronic vehicle administration after the PTZ-preinjection, chronic cyclosporin A administration after the initial injection of PTZ apparently aggravated convulsions after the second PTZ injection. Interestingly, the increase in hippocampal cyclophilin mRNA observed after a single PTZ injection was not found after the second PTZ injection in the group with PTZ pretreatment. Therefore, these findings suggest that cyclosporin A administered peripherally can affect the central nervous system, and that an immune response associated with the first convulsive episode plays a key role in severity during subsequent attacks.",
keywords = "convulsion, cyclophilin, Cyclosporin A, pentylenetetrazol, rat brain, seizures",
author = "Masato Asanuma and Norio Ogawa and Sakiko Nishibayashi and Yoichi Kondo and Akitane Mori",
year = "1995",
month = "1",
doi = "10.1007/BF00995159",
language = "English",
volume = "20",
pages = "101--105",
journal = "Neurochemical Research",
issn = "0364-3190",
publisher = "Springer New York",
number = "1",

}

TY - JOUR

T1 - Effects of repeated injection of cyclosporin A on pentylenetetrazol-induced convulsion and cyclophilin mRNA levels in rat brain

AU - Asanuma, Masato

AU - Ogawa, Norio

AU - Nishibayashi, Sakiko

AU - Kondo, Yoichi

AU - Mori, Akitane

PY - 1995/1

Y1 - 1995/1

N2 - To investigate the relationship between the immune system and convulsions in an animal model, we examined the effects of repeated administration with the immunosuppressant cyclosporin A on pentylenetetrazol (PTZ)-induced convulsions and the changes in the mRNA expression of its binding protein cyclophilin in the rat brain. The consecutive administration of cyclosporin A (5 mg/kg s.c., 14 days) significantly aggravated the severity of convulsions induced with PTZ 75 mg/kg i.p. Furthermore, it down-regulated the levels of cyclophilin mRNA in several brain regions and inhibited the PTZ-induced increase of hippocampal cyclophilin mRNA. Compared with the group without PTZ pretreatment or the group treated with chronic vehicle administration after the PTZ-preinjection, chronic cyclosporin A administration after the initial injection of PTZ apparently aggravated convulsions after the second PTZ injection. Interestingly, the increase in hippocampal cyclophilin mRNA observed after a single PTZ injection was not found after the second PTZ injection in the group with PTZ pretreatment. Therefore, these findings suggest that cyclosporin A administered peripherally can affect the central nervous system, and that an immune response associated with the first convulsive episode plays a key role in severity during subsequent attacks.

AB - To investigate the relationship between the immune system and convulsions in an animal model, we examined the effects of repeated administration with the immunosuppressant cyclosporin A on pentylenetetrazol (PTZ)-induced convulsions and the changes in the mRNA expression of its binding protein cyclophilin in the rat brain. The consecutive administration of cyclosporin A (5 mg/kg s.c., 14 days) significantly aggravated the severity of convulsions induced with PTZ 75 mg/kg i.p. Furthermore, it down-regulated the levels of cyclophilin mRNA in several brain regions and inhibited the PTZ-induced increase of hippocampal cyclophilin mRNA. Compared with the group without PTZ pretreatment or the group treated with chronic vehicle administration after the PTZ-preinjection, chronic cyclosporin A administration after the initial injection of PTZ apparently aggravated convulsions after the second PTZ injection. Interestingly, the increase in hippocampal cyclophilin mRNA observed after a single PTZ injection was not found after the second PTZ injection in the group with PTZ pretreatment. Therefore, these findings suggest that cyclosporin A administered peripherally can affect the central nervous system, and that an immune response associated with the first convulsive episode plays a key role in severity during subsequent attacks.

KW - convulsion

KW - cyclophilin

KW - Cyclosporin A

KW - pentylenetetrazol

KW - rat brain

KW - seizures

UR - http://www.scopus.com/inward/record.url?scp=0028853414&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028853414&partnerID=8YFLogxK

U2 - 10.1007/BF00995159

DO - 10.1007/BF00995159

M3 - Article

C2 - 7739751

AN - SCOPUS:0028853414

VL - 20

SP - 101

EP - 105

JO - Neurochemical Research

JF - Neurochemical Research

SN - 0364-3190

IS - 1

ER -