Effects of recombinase A deficiency on biofilm formation by Streptococcus mutans

S. Inagaki; M. Matsumoto-Nakano; Kazuyo Fujita; K. Nagayama; J. Funao; T. Ooshima

doi:10.1111/j.1399-302X.2008.00480.x

Effects of recombinase A deficiency on biofilm formation by Streptococcus mutans

S. Inagaki, M. Matsumoto-Nakano, Kazuyo Fujita, K. Nagayama, J. Funao, T. Ooshima

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Background/aim: Recombinase A (RecA) is essential for the transformation of both plasmid and chromosomal DNA in Streptococcus pneumoniae and is considered to be related to the SOS-response in Streptococcus mutans. Methods: In the present study, a RecA-deficient mutant strain (RAD) was constructed by insertional inactivation of the recA gene encoding the RecA protein in strain MT8148 of S. mutans, after which the biological functions of acid tolerance and biofilm formation were investigated. Results: RAD showed reduced acid tolerance and produced lower density biofilm compared with the wild-type strain. In addition, confocal microscopic observation indicated that the biofilm produced by RAD was composed of cells with significantly lower viability compared with that produced by strain MT8148. Conclusion: These results suggest that RecA has a relationship with biofilm formation.

Original language	English
Pages (from-to)	104-108
Number of pages	5
Journal	Oral microbiology and immunology
Volume	24
Issue number	2
DOIs	https://doi.org/10.1111/j.1399-302X.2008.00480.x
Publication status	Published - Apr 1 2009
Externally published	Yes

Keywords

Biofilm formation
Recombinase A
Streptococcus mutans

ASJC Scopus subject areas

Microbiology
Immunology
Dentistry(all)
Microbiology (medical)

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10.1111/j.1399-302X.2008.00480.x

Cite this

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abstract = "Background/aim: Recombinase A (RecA) is essential for the transformation of both plasmid and chromosomal DNA in Streptococcus pneumoniae and is considered to be related to the SOS-response in Streptococcus mutans. Methods: In the present study, a RecA-deficient mutant strain (RAD) was constructed by insertional inactivation of the recA gene encoding the RecA protein in strain MT8148 of S. mutans, after which the biological functions of acid tolerance and biofilm formation were investigated. Results: RAD showed reduced acid tolerance and produced lower density biofilm compared with the wild-type strain. In addition, confocal microscopic observation indicated that the biofilm produced by RAD was composed of cells with significantly lower viability compared with that produced by strain MT8148. Conclusion: These results suggest that RecA has a relationship with biofilm formation.",

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AU - Inagaki, S.

AU - Matsumoto-Nakano, M.

AU - Fujita, Kazuyo

AU - Nagayama, K.

AU - Funao, J.

AU - Ooshima, T.

PY - 2009/4/1

Y1 - 2009/4/1

N2 - Background/aim: Recombinase A (RecA) is essential for the transformation of both plasmid and chromosomal DNA in Streptococcus pneumoniae and is considered to be related to the SOS-response in Streptococcus mutans. Methods: In the present study, a RecA-deficient mutant strain (RAD) was constructed by insertional inactivation of the recA gene encoding the RecA protein in strain MT8148 of S. mutans, after which the biological functions of acid tolerance and biofilm formation were investigated. Results: RAD showed reduced acid tolerance and produced lower density biofilm compared with the wild-type strain. In addition, confocal microscopic observation indicated that the biofilm produced by RAD was composed of cells with significantly lower viability compared with that produced by strain MT8148. Conclusion: These results suggest that RecA has a relationship with biofilm formation.

AB - Background/aim: Recombinase A (RecA) is essential for the transformation of both plasmid and chromosomal DNA in Streptococcus pneumoniae and is considered to be related to the SOS-response in Streptococcus mutans. Methods: In the present study, a RecA-deficient mutant strain (RAD) was constructed by insertional inactivation of the recA gene encoding the RecA protein in strain MT8148 of S. mutans, after which the biological functions of acid tolerance and biofilm formation were investigated. Results: RAD showed reduced acid tolerance and produced lower density biofilm compared with the wild-type strain. In addition, confocal microscopic observation indicated that the biofilm produced by RAD was composed of cells with significantly lower viability compared with that produced by strain MT8148. Conclusion: These results suggest that RecA has a relationship with biofilm formation.

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KW - Recombinase A

KW - Streptococcus mutans

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Effects of recombinase A deficiency on biofilm formation by Streptococcus mutans

Abstract

Keywords

ASJC Scopus subject areas

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