Vasoactive intestinal polypeptide (VIP) and growth hormone releasing factor (GRF) stimulated an increase of cAMP accumulation with a concomitant release of PRL and GH, respectively. Release of PRL induced by VIP was partially suppressed by 5 and 25 μM of H-89, whereas VIP-induced gene expression of PRL was inhibited by all concentrations of H-89. Release and gene expression of GH induced by GRF was inhibited by H-89 in a dose dependent manner and completely blocked by 25 μM of H-89. These results indicate that VIP-induced PRL release and gene expression may be mediated, at least in a part, by cAMP-dependent protein kinase pachway, whereas GRF-induced GH release and gene expression may be mediated predominantly by cAMP dependent protein kinase.
|Number of pages||7|
|Journal||Comparative Biochemistry and Physiology - C Pharmacology Toxicology and Endocrinology|
|Publication status||Published - Jan 1 1998|
- mRMNA expression
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