Effects of protein kinase A inhibitor (H-89) on VIP- and GRF-induced release and mRNA expression of prolactin and growth hormone in the chicken pituitary gland

Norio Kansaku, Kiyoshi Shimada, Noboru Saito, Hiroyoshi Hidaka

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Vasoactive intestinal polypeptide (VIP) and growth hormone releasing factor (GRF) stimulated an increase of cAMP accumulation with a concomitant release of PRL and GH, respectively. Release of PRL induced by VIP was partially suppressed by 5 and 25 μM of H-89, whereas VIP-induced gene expression of PRL was inhibited by all concentrations of H-89. Release and gene expression of GH induced by GRF was inhibited by H-89 in a dose dependent manner and completely blocked by 25 μM of H-89. These results indicate that VIP-induced PRL release and gene expression may be mediated, at least in a part, by cAMP-dependent protein kinase pachway, whereas GRF-induced GH release and gene expression may be mediated predominantly by cAMP dependent protein kinase.

Original languageEnglish
Pages (from-to)89-95
Number of pages7
JournalComparative Biochemistry and Physiology - C Pharmacology Toxicology and Endocrinology
Volume119
Issue number1
DOIs
Publication statusPublished - Jan 1 1998
Externally publishedYes

Keywords

  • Forskolin
  • GH
  • GRF
  • H-89
  • PKA
  • PRL
  • VIP
  • cAMP
  • mRMNA expression

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

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