Effects of prostaglandin F and nitric oxide on the secretory function of bovine luteal cells

Anna Korzekwa, Jerzy J. Jaroszewski, Marek Bogacki, Katarzyna M. Deptula, Tomasz S. Maslanka, Tomas J. Acosta, Kiyoshi Okuda, Dariusz J. Skarzynski

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Abstract

The objective of the present study was to investigate the influence of prostaglandin F (PGF) and nitric oxide (NO) on production of steroids and PGs by culturing bovine luteal cells obtained from ovaries on days 8-12 of the estrous cycle with a nitric oxide (NO) donor (Spermine NONOate), and a NO synthase inhibitor (NG-nitro-L-arginine methyl ester dihydrochloride: L-NAME). When the cells were exposed for 24 h to PGP (107-105 M), production of progesterone (P4) increased significantly at all doses used (P<0.05). Moreover, PGP stimulated PGP production (P<0.01), depressed testosterone (T) production (P<0.05), but did not affect synthesis of prostaglandin E2 (PGE2). Spermine NONOate decreased P4 production to 66%, 47% and 34% of the control concentration after treatment with 10-5 M, 10-4 M and 10-3 M, respectively, but did not affect T production, and increased PGF synthesis (P<0.05) and PGP2 (P<0.01) at all doses used. L-NAMP increased production of P4 (P<0.01) but did not affect (P>0.05) secretion of T, PGF and PGP2. Estradiol-17β (E2) was detectable on the level of sensitivity of assay and was not significantly altered by any treatments. The overall results suggest that PGF and NO produced locally in bovine CL play roles in the regulation of the secretory function of the bovine CL as auto/paracrine factors.

Original languageEnglish
Pages (from-to)411-417
Number of pages7
JournalJournal of Reproduction and Development
Volume50
Issue number4
DOIs
Publication statusPublished - Dec 1 2004

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Keywords

  • Corpus luteum
  • Cow
  • Nitric oxide
  • Progesterone
  • Prostaglandins

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Korzekwa, A., Jaroszewski, J. J., Bogacki, M., Deptula, K. M., Maslanka, T. S., Acosta, T. J., Okuda, K., & Skarzynski, D. J. (2004). Effects of prostaglandin F and nitric oxide on the secretory function of bovine luteal cells. Journal of Reproduction and Development, 50(4), 411-417. https://doi.org/10.1262/jrd.50.411