TY - JOUR
T1 - Effects of prostaglandin F2α and nitric oxide on the secretory function of bovine luteal cells
AU - Korzekwa, Anna
AU - Jaroszewski, Jerzy J.
AU - Bogacki, Marek
AU - Deptula, Katarzyna M.
AU - Maslanka, Tomasz S.
AU - Acosta, Tomas J.
AU - Okuda, Kiyoshi
AU - Skarzynski, Dariusz J.
PY - 2004
Y1 - 2004
N2 - The objective of the present study was to investigate the influence of prostaglandin F2α (PGF2α) and nitric oxide (NO) on production of steroids and PGs by culturing bovine luteal cells obtained from ovaries on days 8-12 of the estrous cycle with a nitric oxide (NO) donor (Spermine NONOate), and a NO synthase inhibitor (NG-nitro-L-arginine methyl ester dihydrochloride: L-NAME). When the cells were exposed for 24 h to PGP2α (107-105 M), production of progesterone (P4) increased significantly at all doses used (P<0.05). Moreover, PGP2α stimulated PGP2α production (P<0.01), depressed testosterone (T) production (P<0.05), but did not affect synthesis of prostaglandin E2 (PGE2). Spermine NONOate decreased P4 production to 66%, 47% and 34% of the control concentration after treatment with 10-5 M, 10-4 M and 10-3 M, respectively, but did not affect T production, and increased PGF2α synthesis (P<0.05) and PGP2 (P<0.01) at all doses used. L-NAMP increased production of P4 (P<0.01) but did not affect (P>0.05) secretion of T, PGF 2α and PGP2. Estradiol-17β (E2) was detectable on the level of sensitivity of assay and was not significantly altered by any treatments. The overall results suggest that PGF 2α and NO produced locally in bovine CL play roles in the regulation of the secretory function of the bovine CL as auto/paracrine factors.
AB - The objective of the present study was to investigate the influence of prostaglandin F2α (PGF2α) and nitric oxide (NO) on production of steroids and PGs by culturing bovine luteal cells obtained from ovaries on days 8-12 of the estrous cycle with a nitric oxide (NO) donor (Spermine NONOate), and a NO synthase inhibitor (NG-nitro-L-arginine methyl ester dihydrochloride: L-NAME). When the cells were exposed for 24 h to PGP2α (107-105 M), production of progesterone (P4) increased significantly at all doses used (P<0.05). Moreover, PGP2α stimulated PGP2α production (P<0.01), depressed testosterone (T) production (P<0.05), but did not affect synthesis of prostaglandin E2 (PGE2). Spermine NONOate decreased P4 production to 66%, 47% and 34% of the control concentration after treatment with 10-5 M, 10-4 M and 10-3 M, respectively, but did not affect T production, and increased PGF2α synthesis (P<0.05) and PGP2 (P<0.01) at all doses used. L-NAMP increased production of P4 (P<0.01) but did not affect (P>0.05) secretion of T, PGF 2α and PGP2. Estradiol-17β (E2) was detectable on the level of sensitivity of assay and was not significantly altered by any treatments. The overall results suggest that PGF 2α and NO produced locally in bovine CL play roles in the regulation of the secretory function of the bovine CL as auto/paracrine factors.
KW - Corpus luteum
KW - Cow
KW - Nitric oxide
KW - Progesterone
KW - Prostaglandins
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U2 - 10.1262/jrd.50.411
DO - 10.1262/jrd.50.411
M3 - Article
C2 - 15329472
AN - SCOPUS:16644398287
VL - 50
SP - 411
EP - 417
JO - Journal of Reproduction and Development
JF - Journal of Reproduction and Development
SN - 0916-8818
IS - 4
ER -